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PXD061189

PXD061189 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSurvey of megadalton protein assemblies from Dictyostelium discoideum
DescriptionThe analysis of high-abundance megadalton protein complexes in Dictyostelium discoideum cell lysates was conducted as follows: the solubilized protein fraction was subjected to size-exclusion chromatography isolating fractions corresponding to the megadalton region, followed by ion-exchange chromatography to further resolve the protein complexes. The fractions obtained after both chromatographic steps were then analyzed using mass spectrometry to identify the constituent protein complexes, which provided the necessary data for cryo-EM.
HostingRepositoryMassIVE
AnnounceDate2025-03-12
AnnouncementXMLSubmission_2025-03-12_07:40:02.576.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGabriel Hoogerbrugge
SpeciesList scientific name: Dictyostelium discoideum AX2; NCBI TaxID: 366501;
ModificationListAcetyl; Met-loss; Met-loss+Acetyl
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-25 11:43:07ID requested
12025-03-12 07:40:02announced
Publication List
no publication
Keyword List
submitter keyword: mass spectrometry, Dictyostelium discoideum, cryo-EM, DatasetType:Proteomics
Contact List
Edward Marcotte
contact affiliationUniversity of Texas-Austin
contact emailmarcotte@icmb.utexas.edu
lab head
Gabriel Hoogerbrugge
contact affiliationUniversity of Texas at Austin
contact emailghooger@marcottelab.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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