PXD061187

PXD061187 is an original dataset announced via ProteomeXchange.

Title	Protein-interaction network reveals a role of Prp19 splicing factor in transcription of both intron-containing and intron-less genes
Description	We have previously demonstrated that the transcription-dependent interaction of the promoter and terminator ends of a gene, which results in the formation of a gene loop, is facilitated by the interaction of the general transcription factor TFIIB with the CF1, CPF and Rat1 termination complexes. To further elucidate the protein-protein interactions that stabilize gene loop, we performed mass spectrometry of affinity purified termination complexes from chromatin fraction. Quantitative proteomic analysis revealed additional interactions of termination factors with TFIID and SAGA complex. Since gene looping of intron-containing genes involves additional contacts of the promoter and terminator with the intron, we examined if termination factors interact with the splicing factors as well. All three termination complexes displayed statistically significant interactions with Prp19, Prp43, Sub2, Snu114, Brr2 and Smb1 splicing factors. Since Prp43 and Prp19 consistently emerged as the interactor of both initiation and termination factors, we affinity-purified both and performed mass spectrometry. Prp19 exhibited novel interactions with subunits of TFIID, CPF complex, and the RSC chromatin remodeling complex. These interactions were observed exclusively in the chromatin context, thereby implicating the factor in transcription of protein coding genes. Since fewer than 4% of yeast genes contain introns, we hypothesized that Prp19 might have a broader role in RNAPII transcription cycle. Auxin-mediated depletion of Prp19 resulted in about 25-50% decrease in transcription of both intron-containing and intron-less genes on a genomewide scale. Specifically, the promoter recruitment of TBP registered a significant decline in the absence of Prp19. Chromatin immunoprecipitation (ChIP) analysis revealed crosslinking of Prp19 to the promoter-proximal region of both intronic and non-intronic genes. These findings demonstrate that Prp19 has a novel role in the initiation step of transcription in budding yeast.
HostingRepository	PRIDE
AnnounceDate	2026-02-11
AnnouncementXML	Submission_2026-02-11_15:36:01.688.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061187
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Stemmer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-25 10:25:31	ID requested
⏵ 1	2026-02-11 15:36:02	announced

10.6019/PXD061187;

submitter keyword: RNAPII, chromatin remodeling,yeast, Prp19 splicing factor

Athar Ansari
contact affiliation	Department of Biological Science Wayne State University
contact email	bb2749@wayne.edu
lab head
Paul Stemmer
contact affiliation	Wayne State University
contact email	pmstemmer@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061187
     1. Label: PRIDE project
     2. Name: Protein-interaction network reveals a role of Prp19 splicing factor in transcription of both intron-containing and intron-less genes