PXD061172 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Arylsulfatase L (ARSL) Controls Glycosaminoglycan Sulfation During Skeletal Development |
| Description | Proteoglycans are essential components of the cartilage extracellular matrix (ECM), with glycosaminoglycan (GAG) chains playing a crucial role in skeletal development. This study identifies a previously unknown substrate of arylsulfatase L (ARSL) a Golgi-localized enzyme. To investigate ARSL’s physiological substrate, the study utilized the TurboID proximity labeling approach. TurboID, a biotin ligase, biotinylates interacting proteins, allowing their isolation via streptavidin beads and identification through mass spectrometry (MS). We generated RCS (Rat chondrosarcoma) cells overexpressing FLAG-TurboID-Arsl, confirming its Golgi localization. MS analysis revealed 129 significantly enriched proteins in ARSL-labeled samples, with gene ontology (GO) analysis highlighting extracellular matrix organization. Among these proteins, Acan, a precursor of Aggrecan—rich in sulfated GAGs—was identified as a key ARSL interactor, reinforcing the enzyme’s role in ECM regulation and skeletal development. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-15 |
| AnnouncementXML | Submission_2026-06-14_16:16:29.883.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Marianna Maddaluno |
| SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-02-25 05:23:34 | ID requested | |
| ⏵ 1 | 2026-06-14 16:16:30 | announced | |
Publication List
| Maddaluno M, De Leonibus C, Del Prete E, Salierno FG, Intartaglia D, Carrella D, Conte I, Volpi N, Settembre C, Arylsulfatase L is a Golgi chondroitin sulfatase regulating skeletal development. J Biol Chem, 302(6):113111(2026) [pubmed] |
| 10.1016/j.jbc.2026.113111; |
Keyword List
| submitter keyword: ARSL, Skeletal, Glycosaminoglycan |
Contact List
| Carmine Settembre |
|---|
| contact affiliation | TIGEM |
| contact email | m.maddaluno@tigem.it |
| lab head | |
| Marianna Maddaluno |
|---|
| contact affiliation | Telethon Institute of Genetics and Medicine (TIGEM) |
| contact email | m.maddaluno@tigem.it |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061172
- Label: PRIDE project
- Name: Arylsulfatase L (ARSL) Controls Glycosaminoglycan Sulfation During Skeletal Development
