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PXD061162

PXD061162 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Tumour derived exosomal DDAH1 promotes lung aging and metastasis
Description	Aging is a multifactorial series of molecular alterations that leads to gradual deterioration of tissue function and increased vulnerability to disease and death. Primary tumor communicates with host organs partially through extracellular vesicles. However, the mechanistic drivers of lung aging in the context of cancer remain unclear. Here, we identify cancer cell-secreted dimethylarginine dimethylaminohydrolase-1 (DDAH1) protein induces citrulline accumulation in lung tissue and promotes lung aging. Mechanistically, single cell sequencing and genetic knockout mice evidence that elevation of citrulline availability inhibits peptidylarginine deiminase 4 (PAD4)-mediated transforming growth factor-β (TGF-β1) citrullination, thereby inducing the TGF-β1/Smad3 signaling pathway in lung fibroblasts. Notably, vacuolar protein sorting 35 (VPS35) assists the package of DDAH1 into late endosome. Employing DDAH1 inhibitor PD 404182 treatment reduces lung fibrosis and alleviates lung aging. Conclusively, our finding reveals cancer cell-secreted DDAH1 protein contributes to citrulline accumulation to promote lung aging, shedding light on future applications for the treatment and diagnosis of tumor by clearance of senescent lung fibroblasts.
HostingRepository	iProX
AnnounceDate	2025-10-16
AnnouncementXML	Submission_2025-10-15_18:46:34.718.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liu Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-25 00:55:21	ID requested
⏵ 1	2025-10-15 18:46:35	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: Extracellular vesicles, DDAH1

Contact List

Wei Yan
contact affiliation	Wuhan University
contact email	weiyan@whu.edu.cn
lab head
Liu Liu
contact affiliation	Wuhan University
contact email	862595600@qq.com
dataset submitter

Full Dataset Link List

iProX dataset URI