PXD061156

PXD061156 is an original dataset announced via ProteomeXchange.

Title	The CCL20-integrin α5β1 interaction enhances TGF-β/Smad signaling to promote fibroblast activation and facilitate pulmonary fibrosis
Description	Pulmonary fibrosis (PF) has limited therapeutic options because its molecular pathogenesis remains unclear. Chemokine (C-C motif) ligand 20 (CCL20) plays crucial roles in the pathogenesis of various immune diseases. However, its role in PF is unknown. Here, we showed that CCL20 expression was significantly increased in the lung tissues of PF mouse models and PF patients compared with healthy controls. Type 2 alveolar epithelial cells (AEC2s) were identified as the major producers of CCL20, and increased CCL20 expression resulted from decreased expression of the transcription factor JUN. AEC2-specific deletion of CCL20 protected mice from bleomycin (BLM)-induced PF. Mechanistic studies revealed that CCL20 interacted with integrin α5β1, but not the classical receptor CCR6, on fibroblasts and subsequently enhanced transforming growth factor-β (TGF-β)/Smad signaling, which promoted the differentiation of lung fibroblasts into myofibroblasts. Antibody blockade of CCL20 or disruption of the CCL20-integrin α5β1 interaction reduced established PF. Overall, our study highlights the CCL20-integrin α5β1-TGF-β signaling cascade as a potential therapeutic target for PF.
HostingRepository	iProX
AnnounceDate	2025-02-24
AnnouncementXML	Submission_2025-02-24_19:22:59.079.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minjia Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-24 19:22:43	ID requested
⏵ 1	2025-02-24 19:22:59	announced

Dataset with its publication pending

submitter keyword: Pulmonary fibrosis, Chemokine, CCL20, Integrin α5β1, TGF-β/Smad signaling

Shanshan Liu
contact affiliation	National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University
contact email	lss0625@csu.cn
lab head
Minjia Li
contact affiliation	National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University
contact email	minjiali9604@163.com
dataset submitter

iProX dataset URI