PXD061148

PXD061148 is an original dataset announced via ProteomeXchange.

Title	The ER-phagy receptor FAM134B is targeted by Salmonella Typhimurium to promote infection
Description	Macroautophagy/autophagy is a key catabolic-recycling pathway that can selectively target damaged organelles or invading pathogens for degradation. The selective autophagic degradation of the endoplasmic reticulum (hereafter referred to as ER-phagy) is a homeostatic mechanism, controlling ER size, the removal of misfolded protein aggregates, and organelle damage. ER-phagy can also be stimulated by pathogen infection. However, the link between ER-phagy and bacterial infection remains poorly understood, as are the mechanisms evolved by pathogens to escape the effects of ER-phagy. Here, we show that Salmonella enterica serovar Typhimurium inhibits ER-phagy by targeting the ER-phagy receptor FAM134B, leading to a pronounced increase in Salmonella burden after invasion. Salmonella prevents FAM134B oligomerization, which is required for efficient ER-phagy. FAM134B knock-out raises intracellular Salmonella number, while FAM134B activation reduces Salmonella burden. Additionally, we found that Salmonella targets FAM134B through the bacterial effector SopF to enhance intracellular survival through ER-phagy inhibition. Furthermore, FAM134B knock-out mice infected with Salmonella presented severe intestinal damage and increased bacterial burden. These results provide mechanistic insight into the interplay between ER-phagy and bacterial infection, highlighting a key role for FAM134B in innate immunity.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_07:26:51.888.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061148
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ryan Russell
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Salmonella enterica subsp. enterica; NCBI TaxID: NCBITaxon:59201;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-24 14:13:56	ID requested
⏵ 1	2025-05-07 07:26:52	announced

Gatica D, Alsaadi RM, El Hamra R, Li B, Mueller R, Miyazaki M, Sun Q, Sad S, Russell RC, The ER-phagy receptor FAM134B is targeted by Salmonella Typhimurium to promote infection. Nat Commun, 16(1):2923(2025) [pubmed]

10.1038/s41467-025-58035-7;

10.6019/PXD061148;

submitter keyword: AP-MS SopF FAM134B

Ryan C Russell
contact affiliation	Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
contact email	ryan.russell@uottawa.ca
lab head
Ryan Russell
contact affiliation	University of Ottawa
contact email	ryan.russell@uottawa.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061148
     1. Label: PRIDE project
     2. Name: The ER-phagy receptor FAM134B is targeted by Salmonella Typhimurium to promote infection