PXD061148 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The ER-phagy receptor FAM134B is targeted by Salmonella Typhimurium to promote infection |
Description | Macroautophagy/autophagy is a key catabolic-recycling pathway that can selectively target damaged organelles or invading pathogens for degradation. The selective autophagic degradation of the endoplasmic reticulum (hereafter referred to as ER-phagy) is a homeostatic mechanism, controlling ER size, the removal of misfolded protein aggregates, and organelle damage. ER-phagy can also be stimulated by pathogen infection. However, the link between ER-phagy and bacterial infection remains poorly understood, as are the mechanisms evolved by pathogens to escape the effects of ER-phagy. Here, we show that Salmonella enterica serovar Typhimurium inhibits ER-phagy by targeting the ER-phagy receptor FAM134B, leading to a pronounced increase in Salmonella burden after invasion. Salmonella prevents FAM134B oligomerization, which is required for efficient ER-phagy. FAM134B knock-out raises intracellular Salmonella number, while FAM134B activation reduces Salmonella burden. Additionally, we found that Salmonella targets FAM134B through the bacterial effector SopF to enhance intracellular survival through ER-phagy inhibition. Furthermore, FAM134B knock-out mice infected with Salmonella presented severe intestinal damage and increased bacterial burden. These results provide mechanistic insight into the interplay between ER-phagy and bacterial infection, highlighting a key role for FAM134B in innate immunity. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_07:26:51.888.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD061148 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ryan Russell |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Salmonella enterica subsp. enterica; NCBI TaxID: NCBITaxon:59201; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-24 14:13:56 | ID requested | |
⏵ 1 | 2025-05-07 07:26:52 | announced | |
Publication List
Gatica D, Alsaadi RM, El Hamra R, Li B, Mueller R, Miyazaki M, Sun Q, Sad S, Russell RC, The ER-phagy receptor FAM134B is targeted by Salmonella Typhimurium to promote infection. Nat Commun, 16(1):2923(2025) [pubmed] |
10.1038/s41467-025-58035-7; |
10.6019/PXD061148; |
Keyword List
submitter keyword: AP-MS SopF FAM134B |
Contact List
Ryan C Russell |
contact affiliation | Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada. |
contact email | ryan.russell@uottawa.ca |
lab head | |
Ryan Russell |
contact affiliation | University of Ottawa |
contact email | ryan.russell@uottawa.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061148 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061148
- Label: PRIDE project
- Name: The ER-phagy receptor FAM134B is targeted by Salmonella Typhimurium to promote infection