PXD061145

PXD061145 is an original dataset announced via ProteomeXchange.

Title	Defining enteric bacterial pathogenesis using organoids: Citrobacter rodentium uses EspC, an atypical mucinolytic protease, to penetrate mouse colonic mucus
Description	Enteric bacterial pathogens pose significant threats to human health; however, the mechanisms by which they infect the mammalian gut in the face of daunting host defenses remain to be fully defined. For the attaching and effacing (A/E) bacterial family member and murine pathogen Citrobacter rodentium, its virulence strategy appears to involve penetration of the colonic mucus barrier to reach the underlying epithelium. To better define these interactions, we grew colonoids under air-liquid interface (ALI) conditions, producing a thick mucus layer that mimicked in vivo mucus composition and glycosylation. C. rodentium’s penetration of ALI-derived mucus was dramatically enhanced upon exposure to sialic acid, in concert with the secretion of two autotransporter (SPATE) proteins, Pic and EspC. Despite Pic being a class II SPATE, and already recognized as a mucinase, it was EspC, a class I SPATE family member, that degraded ALI-derived mucus, despite class I SPATES not previously shown to possess mucinase activity. Confirming this finding, E. coli DH5a carrying a plasmid that expresses C. rodentium-derived EspC was able to degrade the mucus. Moreover, recombinant EspC alone also displayed mucinolytic activity in a dose-dependent manner. Collectively, our results reveal the utility of ALI-derived mucus in modeling microbe-host interactions at the intestinal mucosal surface, as well as identify EspC as an atypical class I SPATE that shows significant mucinolytic activity towards ALI-derived mucus.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-16_16:12:47.407.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Moon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-24 12:32:13	ID requested
⏵ 1	2026-08-16 16:12:48	announced

10.1080/19490976.2025.2494717;

Chen Y, Gilliland A, Liang Q, Han X, Yang H, Chan J, L, é, vesque D, Moon KM, Daneshgar P, Boisvert FM, Foster L, Zandberg WF, Bergstrom K, Yu HB, Vallance BA, uses EspC, an atypical mucinolytic protease, to penetrate mouse colonic mucus. Gut Microbes, 17(1):2494717(2025) [pubmed]

submitter keyword: EspC, mucus, Air-liquid interface, colonoids,Enteric pathogens

Leonard J. Foster
contact affiliation	Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Canada
contact email	foster@msl.ubc.ca
lab head
Jenny Moon
contact affiliation	University of British Columbia
contact email	kyungmee@mail.ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD061145

PRIDE project URI

• PRIDE
  1. PXD061145
     1. Label: PRIDE project
     2. Name: Defining enteric bacterial pathogenesis using organoids: Citrobacter rodentium uses EspC, an atypical mucinolytic protease, to penetrate mouse colonic mucus