PXD061136

PXD061136 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins interacting with GPAT4 in HUVECs cell line.
Description	The endocardium plays a pivotal role in governing myocardial development, and understanding the intrinsic regulatory insights will help apprehend pathological cardiomyopathy. Glycerol-3-phosphate acyltransferase 4 (GPAT4) is an endoplasmic reticulum (ER) membrane anchored protein. While the role of GPAT4 in glycerophospholipid biosynthesis is well established, its function in the ER is less explored. Here, we generate Gpat4 global and tissue-specific knockout mice and identify the essential role of GPAT4 in endocardial development. Deficiency of GPAT4 provokes endocardial ER stress response and enhances ER-mitochondrial (ER-mito) communications, leading to mitochondrial DNA (mtDNA) escape. As a result, the cGAS-STING pathway triggers to stimulate type-I-interferon response, which affects heart development. Finally, abolishment of the cGAS-STING-type-I-interferon pathway rescues the heart defects of Gpat4 deletion mice. These findings uncover the pivotal role of GPAT4 in the maintenance of ER homeostasis during endocardial and heart development. Meanwhile, this study highlights the importance of the cGAS-STING pathway in cardiac organogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_07:26:39.631.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	chengzhi wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-24 06:45:29	ID requested
⏵ 1	2025-05-07 07:26:40	announced

Zhao T, Jin K, Wang X, Su X, Wang Y, Gao M, Luo W, Yang H, Yang Z, GPAT4 sustains endoplasmic reticulum homeostasis in endocardial cells and safeguards heart development. Nat Commun, 16(1):3345(2025) [pubmed]

10.1038/s41467-025-58722-5;

submitter keyword: GPAT4, ER stress,HUVECs

Lei Fang
contact affiliation	Medical School of Nanjing University
contact email	njfanglei@nju.edu.cn
lab head
chengzhi wang
contact affiliation	university
contact email	602022350046@smail.nju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061136

PRIDE project URI

• PRIDE
  1. PXD061136
     1. Label: PRIDE project
     2. Name: Identification of proteins interacting with GPAT4 in HUVECs cell line.