PXD061133

PXD061133 is an original dataset announced via ProteomeXchange.

Title	Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis
Description	Heat stress inhibits photosynthesis efficiency, thereby suppressing plant growth and crop yield. However, the mechanism underlying this inhibition is not fully understood. Here, we report that the multiple organellar RNA-editing factor 8 (MORF8) forms condensates with solid-like properties in chloroplast upon heat stress. In vitro data show that the MORF8 condensation is intrinsically heat-dependent and primarily determined by its IDR (intrinsically disordered region). Purification and characterization of MORF8 condensates show that numerous editing factors including PPR proteins and MORFs are partitioned. We provide both genetic and biochemical evidence that MORF8 condensation inhibits chloroplast RNA editing. In agreement, we find that both heat stress and MORF8 condensation lead to reduced editing of RNAs encoding NADH dehydrogenase-like (NDH) complex and impaired NDH activity and photosynthesis efficiency. These findings uncover MORF8 as a putative chloroplastic thermosensor that mediates photosynthesis inhibition by heat and highlight the functional significance of solid material properties of biomolecular condensates.
HostingRepository	PRIDE
AnnounceDate	2025-03-07
AnnouncementXML	Submission_2025-03-06_20:51:41.430.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061133
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jie Wu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-24 05:42:23	ID requested
⏵ 1	2025-03-06 20:51:41	announced

10.6019/PXD061133;

submitter keyword: phase seperation,Arabidopsis thaliana， RNA editing

Xiaofeng Fang
contact affiliation	1Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
contact email	xffang@tsinghua.edu.cn
lab head
Jie Wu
contact affiliation	Tsinghua University
contact email	daisywujie@mail.tsinghua.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061133
     1. Label: PRIDE project
     2. Name: Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis