PXD061125

PXD061125 is an original dataset announced via ProteomeXchange.

Title	Identification of potential self-interaction sites on golgin Imh1-C213 by cross-linking mass spectrometry
Description	The golgins are long coiled-coil proteins involved in vesicular transport to the Golgi, a process that contributes to Golgi function and integrity. Previous studies have elucidated that their self-interaction and their interaction with small GTPase Arl1 are critical for their Golgi localization. Here, we report that glycerol regulates Golgi localization of Imh1, a prototypic yeast golgin. Experimentally, we observed that cellular conditions leading to reduced intracellular glycerol levels trigger the release of Imh1 from the Golgi, whereas restoring glycerol levels reverses this effect. Given that glycerol is a well-documented molecular chaperone, we investigated its impact on the conformation of the Imh1 C-terminal fragment (Imh1-C213) in vitro, employing limited proteolysis assays. To further understand glycerol-induced conformational changes, we used isotope-labeled BS3-d0/d4 crosslinkers in combination with LC-MS/MS to map potential self-interaction sites on Imh1-C213. Our findings reveal and quantify several distinct cross-linked peptide pairs within Imh1-C213, in both the presence and absence of glycerol. These observed differences likely correspond to glycerol-induced conformational changes, which provide novel insights into the structural dynamics of golgins and their functional regulation within the Golgi apparatus.
HostingRepository	PRIDE
AnnounceDate	2025-04-30
AnnouncementXML	Submission_2025-04-30_00:01:38.008.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chia-Jung Yu
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-24 01:35:00	ID requested
⏵ 1	2025-04-30 00:01:38	announced

Dataset with its publication pending

submitter keyword: golgin, Golgi, dimer, cross-link, glycerol,Imh1

Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@gmail.com
lab head
Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@mail.cgu.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061125
     1. Label: PRIDE project
     2. Name: Identification of potential self-interaction sites on golgin Imh1-C213 by cross-linking mass spectrometry