PXD061123

PXD061123 is an original dataset announced via ProteomeXchange.

Title	Macrophages promote collagen fibrillogeneis through modulation of fibroblast circadian rhythms
Description	Collagen deposition in fibroblasts, the primary collagen-producing cells, is regulated by both macrophages and the circadian rhythm, although how these regulatory processes interact with one another is unknown. Here, we reveal that macrophage-fibroblast interaction enhances collagen deposition which requires a functional circadian rhythm. This response is also dependent on macrophage polarisation status, where M0 (naïve) and M1 (pro-inflammatory) macrophages require direct cell-cell contact; whereas M2 (anti-inflammatory) macrophages utilise an additional mechanism through secreted soluble factors that strengthen circadian rhythms and significantly increase collagen deposition in fibroblasts, independent of cell-cell contact. Using mass spectrometry proteomics analysis, we identified PDGFA as a key factor in induction of circadian amplitude in fibroblasts.
HostingRepository	PRIDE
AnnounceDate	2026-08-07
AnnouncementXML	Submission_2026-08-06_20:04:47.113.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joan Chang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-24 00:54:52	ID requested
⏵ 1	2026-08-06 20:04:47	announced

Dataset with its publication pending

submitter keyword: Thp1, circadian rhythm, polarisation, macrophages, collagen, secretome

Joan Chang
contact affiliation	Manchester Cell-Matrix Centre, Division of Molecular and Cellular Function, FBMH, University of Manchester
contact email	joan.chang@manchester.ac.uk
lab head
Joan Chang
contact affiliation	University of Manchester
contact email	joan.chang86@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD061123

PRIDE project URI

• PRIDE
  1. PXD061123
     1. Label: PRIDE project
     2. Name: Macrophages promote collagen fibrillogeneis through modulation of fibroblast circadian rhythms