PXD061096

PXD061096 is an original dataset announced via ProteomeXchange.

Title	Anticancer Effects of DNA-Functionalized Gold Nanoparticle–Mediated Photothermal Therapy
Description	Vascular endothelial growth factor (VEGF)-targeted therapies are promising in inhibiting tumor angiogenesis but often face limitations due to resistance and incomplete efficacy. Combining VEGF inhibition with photothermal therapy (PTT), which uses nanoparticles such as gold nanoparticles to generate heat upon light exposure, offers a potential solution by targeting both the tumor vasculature and the tumor cells directly. However, the molecular mechanisms driving the synergy between these treatments remain unclear. In this study we employed quantitative proteomics to investigate the molecular alterations induced by combining VEGF-targeted aptamers with PTT in MCF-7 breast cancer cells. Our proteomic analysis revealed significant changes in proteins involved in cell cycle regulation, RNA splicing, and stress responses, particularly those associated with p53 activation and endoplasmic reticulum stress. Specifically, the combined treatment suppressed cell cycle progression, activated endoplasmic reticulum stress, and inhibited stress granule disassembly, leading to increased tumor cell death. These findings provide new insights into the therapeutic mechanisms of this combination therapy and also demonstrate the general potential of quantitative proteomics in elucidating complex treatment effects. Furthermore, this study highlights how proteomics can help identify biomarkers for treatment efficacy and guide the optimization of combination therapies for cancer.
HostingRepository	PRIDE
AnnounceDate	2025-10-15
AnnouncementXML	Submission_2025-10-15_03:02:45.862.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Young Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-23 09:21:18	ID requested
⏵ 1	2025-10-15 03:02:46	announced

10.1016/J.BBRC.2025.152625;

submitter keyword: VEGF, mass spectrometry, photothermal therapy,DNA-functionalized gold nanoparticles, quantitative proteomics

Jin Gyeong Son
contact affiliation	Nanobio Measurement Group, Division of Biomedical Metrology, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea
contact email	yeskyoung@kriss.re.kr
lab head
Sun Young Lee
contact affiliation	Korea Research Institute of Standards and Science
contact email	sylee12@kriss.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061096
     1. Label: PRIDE project
     2. Name: Anticancer Effects of DNA-Functionalized Gold Nanoparticle–Mediated Photothermal Therapy