PXD061090

PXD061090 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Data-independent acquisition proteomics of rat serum between LIPUS and OA
Description	Previous studies have reported that low-intensity pulsed ultrasound (LIPUS) can alleviate cartilage degradation in osteoarthritis (OA). However, the functions and mechanisms of LIPUS in synovial fibrosis with OA require further study. To investigate the role of the PI3K/AKT signaling pathway in synovial fibrosis and in LIPUS treatment in synovial fibrosis, a TGF-stimulated rat FLS cell model and a rat OA animal model based on anterior cruciate ligament transection (ACLT) and partial medial meniscectomy (MMx) were used. The results revealed that LIPUS delayed the progression of OA. Masson staining revealed that LIPUS reduced the collagen deposition of synovial tissue in OA rats. Correspondingly, immunofluorescence demonstrated that LIPUS significantly downregulated the expression of α-SMA and Col3a1 in OA rats. Moreover, TGF-β stimulation upregulated fibrosis markers at the mRNA and protein levels in FLS, as well as increased phosphorylation-dependent activation of the PI3K/Akt pathway. 740Y-P was found to promote the fibrotic change of FLS induced by TGF-β, but LY294002 reduced its expression. However, LIPUS inhibits the fibrotic change and activation of the PI3K/Akt pathway in FLS under stimulation of TGF-β. In conclusion, LIPUS alleviates synovial fibrosis by blocking the PI3K/AKT pathway.
HostingRepository	PRIDE
AnnounceDate	2025-03-23
AnnouncementXML	Submission_2025-03-23_03:53:25.499.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qing Liao
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-22 23:34:49	ID requested
⏵ 1	2025-03-23 03:53:26	announced

Publication List

10.1038/s41598-025-92413-x;
Liao Q, Chen J, Liu G, Low intensity pulsed ultrasound alleviates synovial fibrosis in osteoarthritis via the PI3K/AKT pathway. Sci Rep, 15(1):9644(2025) [pubmed]

10.1038/s41598-025-92413-x;

Liao Q, Chen J, Liu G, Low intensity pulsed ultrasound alleviates synovial fibrosis in osteoarthritis via the PI3K/AKT pathway. Sci Rep, 15(1):9644(2025) [pubmed]

Keyword List

submitter keyword: rat
serum
Data-independent acquisition proteomics

submitter keyword: rat

serum

Data-independent acquisition proteomics

Contact List

Gang Liu
contact affiliation	Department of Rehabilitation Medicine, Nanfang Hospital of Southern Medical University, Guangzhou 510000, China.
contact email	lg2781@smu.edu.cn
lab head
Qing Liao
contact affiliation	17819576220
contact email	lq12181202@163.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD061090
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD061090

PRIDE project URI

Repository Record List

[ + ]