PXD061082

PXD061082 is an original dataset announced via ProteomeXchange.

Title	Enhancing therapeutic efficacy through degradation of endogenous extracellular matrix in primary breast tumor spheroids.
Description	Cancer remains the leading cause of death globally, with nearly 20 million new cases and 10 million fatalities in 2022. The majority (90%) are solid tumors, which are strongly influenced by the tumor microenvironment (TME)—a complex network of blood vessels, connective tissues, immune cells, and the extracellular matrix (ECM). The ECM, rich in collagen, fibronectin, elastin, and laminin, is constantly remodelled, driving tumor progression and drug resistance while increasing tissue stiffness. Breast cancer, the most common malignancy in women, causes 500,000 deaths annually. Its ECM, particularly collagen composition, varies depending on tumor type and prognosis. Recent research has emphasized 3D tumor spheroid models, which replicate in vivo tumor conditions better than traditional 2D cultures. These models allow more accurate drug testing and better understanding of tumor biology. To improve treatment, researchers have developed a 3D breast cancer spheroid model using rat-derived primary cells with an endogenous ECM structure. By applying collagenases to break down ECM barriers, they demonstrated that ECM degradation enhances drug penetration and chemotherapy effectiveness. This study highlights the critical role of ECM in cancer progression and treatment resistance. Targeting the ECM within advanced 3D models could improve therapeutic outcomes and lead to more effective anticancer strategies.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:25:56.909.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Margot Lo Pinto
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-22 10:35:12	ID requested
⏵ 1	2025-07-13 16:25:57	announced

10.1111/febs.70069;

Lo Cicero A, Campora S, Lo Buglio G, Cin, à P, Lo Pinto M, Scilabra SD, Ghersi G, Enhancing therapeutic efficacy through degradation of endogenous extracellular matrix in primary breast tumor spheroids. FEBS J, 292(13):3494-3507(2025) [pubmed]

submitter keyword: Collagen, 3D Spheroids, Breast Cancer, Extracellular Matrix (ECM), Drug Penetration,Tumor Microenvironment (TME)

Simone Dario Scilabra
contact affiliation	Fondazione Ri.MED
contact email	sdscilabra@fondazionerimed.com
lab head
Margot Lo Pinto
contact affiliation	Fondazione Ri.MED
contact email	mlopinto@fondazionerimed.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD061082

PRIDE project URI

• PRIDE
  1. PXD061082
     1. Label: PRIDE project
     2. Name: Enhancing therapeutic efficacy through degradation of endogenous extracellular matrix in primary breast tumor spheroids.