PXD061072

PXD061072 is an original dataset announced via ProteomeXchange.

Title	Development and Validation of a Novel LC-MS/MS Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR)
Description	Retinol binding protein 4 (RBP4) is the plasma carrier of retinol that complexes with transthyretin (TTR). A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous absolute quantitation of serum and plasma RBP4 and TTR is needed to advance the understanding of RBP4 and TTR as biomarkers. Surrogate peptides with reproducible, linear LC-MS/MS response were selected for RBP4 and TTR quantitation. Purified proteins were used as quantitation standards and heavy labelled peptides as internal standards. Matrix effects were evaluated for quantitation. The method was validated using pooled human serum and applied to measure inter- and intra-individual variability in RBP4 and TTR concentrations in healthy individuals and in patients with diabetic kidney disease. The assay inter-day variability was <12% and precision within 5%. RBP4 and TTR quantitation correlated with commercially available ELISA assays.
HostingRepository	PanoramaPublic
AnnounceDate	2025-09-26
AnnouncementXML	Submission_2025-09-26_08:22:34.457.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nina Isoherranen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4); Label:13C(9)15N(1)
Instrument	QTRAP 5500

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-21 18:39:35	ID requested
⏵ 1	2025-09-26 08:22:35	announced

Yadav AS, Rubinow KB, Zelter A, Authement AK, Kestenbaum BR, Amory JK, Isoherranen N, Development and Validation of a Novel LC-MS/MS-Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR). J Proteome Res, 24(10):5266-5276(2025) [pubmed]

submitter keyword: Retinol binding protein 4 (RBP4), transthyretin (TTR), vitamin A, proteomics, method development, biomarkers

Nina Isoherranen
contact affiliation	University of Washington
contact email	ni2@uw.edu
lab head
Nina Isoherranen
contact affiliation	University of Washington
contact email	ni2@uw.edu
dataset submitter

Panorama Public dataset URI