PXD061064

PXD061064 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Regulation of lipid metabolism gene expression in the C. elegans nervous system occurs via altering the repertoire of RNA binding proteins present on specific transcripts
Description	Tissue-specific regulation of gene expression is essential for multicellular organisms, and RNA binding proteins play central roles in these molecular processes. To determine how the Caenorhabditis elegans RNA binding protein ADR-1 regulates tissue-specific gene expression, we profiled the binding targets of ADR-1 in neural cells and assessed the effects of ADR-1 RNA binding on neural gene expression. We identified a cohort of neural transcripts that facilitate lipid synthesis and are directly regulated by ADR-1 RNA binding. To identify cellular factors that influence ADR-1 binding, a forward genetic screen was performed, revealing that the serine/threonine protein kinase, glycogen synthase kinase-3 (GSK-3), inhibits ADR-1 binding to the cohort. Further investigation revealed that RNA binding protein VIG-1, promotes ADR-1 binding to the cohort in a GSK-3-dependent manner. Together, we reveal that interplay between kinases and RNA binding proteins regulates expression of lipid metabolism genes within neural cells, potentially impacting stress resistance and longevity.
HostingRepository	MassIVE
AnnounceDate	2025-02-21
AnnouncementXML	Submission_2025-02-21_11:20:16.347.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emma Doud
SpeciesList	scientific name: Caenorhabditis elegans; common name: roundworm; NCBI TaxID: 6239;
ModificationList	Oxidation; Phospho; Carbamidomethyl
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-21 11:14:39	ID requested
⏵ 1	2025-02-21 11:20:16	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: ADR-1, glycogen synthase kinase-3 (GSK-3), Caenorhabditis elegans, lipid synthesis, stress resistance, RNA binding protein, DatasetType:Proteomics

submitter keyword: ADR-1, glycogen synthase kinase-3 (GSK-3), Caenorhabditis elegans, lipid synthesis, stress resistance, RNA binding protein, DatasetType:Proteomics

Contact List

Heather Hundley
contact affiliation	Indiana University
contact email	hahundle@iu.edu
lab head
Amber L. Mosley
contact affiliation	Indiana University School of Medicine
contact email	almosley@iu.edu
lab head
Emma Doud
contact affiliation	IUSM
contact email	edoud@iu.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v09/MSV000097188/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v09/MSV000097188/