PXD061053

PXD061053 is an original dataset announced via ProteomeXchange.

Title	Exploring proteome perturbations by citicoline treament
Description	This project deals with an investigation into perturbations of intracellular proteome of SH-SY5Y cells stimulated with two doses of citicoline (1 mM and 0.1 mM) for 6h and 18h in vitro. Citicoline is a nootropic agent used for the therapy of glaucoma, which is a neurodegenerative disorder characterized by degeneration of retinal ganglion cells and the optic nerve. Citicoline is an endogenous metabolite and synthetic precursor of phospholipids and neurotransmitters, thereby acting as a neuroenhancer. Over the last decade, several studies have pointed out neuroprotective activities of citicoline in different preclinical models. However, the molecular mechanisms underscoring citicoline neuroprotection have never been characterized yet. Herein, we set up an LFQ shot gun proteomic approach to address whether citicoline stimulation induces proteome alteration which could help explain its neuroprotective activities. The study strongly supports the hypothesis that citicoline treatment triggers a robust remodelling of intracellular proteome, potentiation the intracellular mechanisms deputed to proteostasis regulation, mitochondrial metabolism, together with transcription, mRNA splicing and protein translation. On the basis of these data, we propose that citicoline may promote proteostasis through processes referred to as hormesis.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_17:42:05.484.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061053
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Diego Sbardella
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-21 07:27:07	ID requested
⏵ 1	2026-02-01 17:42:06	announced

10.6019/PXD061053;

10.3390/pharmaceutics18010061;

Cavaterra D, Giammaria S, Pandino I, Zingale GA, Delli Paoli V, Fiore R, Michelessi M, Roberti G, Carnevale C, Tanga L, Cazzato D, Peroni E, Grasso G, Manni G, Bocedi A, Oddone F, Coletta M, Sbardella D, Tundo GR, Citicoline Triggers Proteome Remodeling and Proteostatic Adaptation: Evidence from Shotgun Proteomics. Pharmaceutics, 18(1):(2026) [pubmed]

submitter keyword: Citicoline, glaucoma, proteostasis

Diego Sbardella
contact affiliation	IRCCS Fondazione Bietti, Rome, Italy
contact email	diego.sbardella@fondazionebietti.it
lab head
Diego Sbardella
contact affiliation	IRCCS Fondazione Bietti
contact email	diego.sbardella@fondazionebietti.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD061053

PRIDE project URI

• PRIDE
  1. PXD061053
     1. Label: PRIDE project
     2. Name: Exploring proteome perturbations by citicoline treament