PXD061021 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular Structure and Enzymatic Mechanism of the Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1 |
Description | During collagen biosynthesis, lysine residues undergo extensive post-translational modifications through the alternate action of two distinct metal ion-dependent enzyme families (i.e., LH/PLODs and GLT25D/COLGALT), ultimately producing the highly conserved alpha-(1,2)-glucosyl-beta-(1,2)-galactosyl-5-hydroxylysine pattern. Malfunctions in these enzymes are linked to developmental pathologies and extracellular matrix alterations associated to enhanced aggressiveness of solid tumors. Here, we characterized human GLT25D1/COLGALT1, revealing an elongated head-to-head homodimeric assembly. Each monomer encompasses two domains (GT1 and GT2), both unexpectedly capable of binding metal ion cofactors and UDP-alpha-galactose donor substrates, resulting in four candidate catalytic sites per dimer. We identified the catalytic site in GT2, featuring an unusual Glu-Asp-Asp motif critical for Mn2+ binding, ruling out direct catalytic roles for the GT1 domain, but showing that the unexpectedly bound Ca2+ and UDP-alpha-galactose cofactors are critical for folding stability. Dimerization was not essential for GLT25D1/COLGALT1 activity, but rather a hallmark for multi-enzyme assembly interactions and/or collagen substrate recognition. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-05 |
AnnouncementXML | Submission_2025-03-05_02:17:40.921.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD061021 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alice Vetrano |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-20 14:09:01 | ID requested | |
⏵ 1 | 2025-03-05 02:17:41 | announced | |
Publication List
Keyword List
submitter keyword: Glycosyltransferase,Collagen Biosynthesis |
Extracellular Matrix |
Post-translational modifications |
Contact List
Claudio Iacobucci |
contact affiliation | University of L'Aquila |
contact email | claudio.iacobucci@univaq.it |
lab head | |
Alice Vetrano |
contact affiliation | University of L'Aquila |
contact email | alice.vetrano@univaq.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061021
- Label: PRIDE project
- Name: Molecular Structure and Enzymatic Mechanism of the Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1