PXD061002

PXD061002 is an original dataset announced via ProteomeXchange.

Title	Neurochondrin Drives Colorectal Cancer Progression by Modulating the PODXL–Ezrin Axis and Mitochondrial Function
Description	Neurochondrin (NCDN) has been recently identified as upregulated in metastatic to liver KM12SM colorectal cancer (CRC) cells in comparison to the poorly metastatic KM12C CRC cells. Furthermore, high levels of NCDN in patients correlate to a worse survival. In this work, using an independent patient cohort, we validated the worsened prognosis associated to NCDN’s elevated levels. Then, we sought to investigate the role of NCDN in CRC progression using the isogenic KM12 CRC cell model comprised of the aforementioned KM12C and KM12SM cells. Stable silencing of NCDN in both cell lines produced a reduction on the tumorigenic capacities of the cells in vitro as seen through different assays (proliferation, adhesion, colony formation…). In vivo, mice injected with the silenced cells had impaired tumor growth and metastasis compared to mice injected with the control cells. Proteomic profiling of NCDN-silenced cells uncovered a set of dysregulated proteins associated with NCDN such as PODXL, indicating that the NCDN-PODXL axis constitutes a critical mediator of CRC liver metastasis. Our findings position NCDN and their associated dysregulated proteins as drivers of metastatic progression in CRC.
HostingRepository	PRIDE
AnnounceDate	2026-05-03
AnnouncementXML	Submission_2026-05-03_06:44:03.206.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Montero Calle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-20 02:18:17	ID requested
⏵ 1	2026-05-03 06:44:03	announced

10.1038/S41419-026-08747-5;

submitter keyword: None

Rodrigo Barderas
contact affiliation	Chronic Disease Programme (UFIEC), Instituto de Salud Carlos III, Madrid, Spain.
contact email	r.barderasm@isciii.es
lab head
Ana Montero Calle
contact affiliation	Instituto de Salud Carlos III
contact email	ana.monteroc@isciii.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD061002

PRIDE project URI

• PRIDE
  1. PXD061002
     1. Label: PRIDE project
     2. Name: Neurochondrin Drives Colorectal Cancer Progression by Modulating the PODXL–Ezrin Axis and Mitochondrial Function