PXD060997 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An atypical Arp2/3 complex is required for Plasmodium DNA segregation and transmission of malaria. |
Description | Plasmodium parasites, the causative agents of malaria, undergo crucial developments within the mosquito vector, initiated by the formation of male and female gametes from gametocytes. Male gametogenesis involves three rapid rounds of endomitosis followed by a single round of DNA segregation and nuclear division during gamete budding. How the cell organises the segregation of eight genomes from a single octoploid nucleus into eight haploid gametes is currently unknown. Here, we discovered an atypical five-subunit Arp2/3 complex in Plasmodium important for correct DNA segregation during male gametogenesis. Unlike the canonical seven-subunit Arp2/3 complex found in other eukaryotes, Plasmodium Arp2/3 localizes to endomitotic spindles and interacts with a kinetochore-associated protein. Disruption of key Arp2/3 subunits or actin polymerization interferes with kinetochore-spindle association, resulting in the formation of sub-haploid gametes and halting transmission. Our work identified an evolutionary divergent Arp2/3 protein complex in malaria parasites, offers novel insights into gametogenesis, and uncovers potential targets for transmission-blocking interventions. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-22 |
AnnouncementXML | Submission_2025-04-22_05:16:42.488.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marcin Luzarowski |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Plasmodium berghei; NCBI TaxID: 5821; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-19 23:46:57 | ID requested | |
⏵ 1 | 2025-04-22 05:16:43 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Arp2/3 complex, chromosome segregation,Malaria, Plasmodium |
Contact List
Marcin Luzarowski |
contact affiliation | Core Facility for Mass Spectrometry and Proteomics, Center for Molecular Biology of Heidelberg University (ZMBH) |
contact email | m.luzarowski@zmbh.uni-heidelberg.de |
lab head | |
Marcin Luzarowski |
contact affiliation | University of Heidelberg |
contact email | m.luzarowski@zmbh.uni-heidelberg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060997
- Label: PRIDE project
- Name: An atypical Arp2/3 complex is required for Plasmodium DNA segregation and transmission of malaria.