PXD060997

PXD060997 is an original dataset announced via ProteomeXchange.

Title	An atypical Arp2/3 complex is required for Plasmodium DNA segregation and transmission of malaria.
Description	Plasmodium parasites, the causative agents of malaria, undergo crucial developments within the mosquito vector, initiated by the formation of male and female gametes from gametocytes. Male gametogenesis involves three rapid rounds of endomitosis followed by a single round of DNA segregation and nuclear division during gamete budding. How the cell organises the segregation of eight genomes from a single octoploid nucleus into eight haploid gametes is currently unknown. Here, we discovered an atypical five-subunit Arp2/3 complex in Plasmodium important for correct DNA segregation during male gametogenesis. Unlike the canonical seven-subunit Arp2/3 complex found in other eukaryotes, Plasmodium Arp2/3 localizes to endomitotic spindles and interacts with a kinetochore-associated protein. Disruption of key Arp2/3 subunits or actin polymerization interferes with kinetochore-spindle association, resulting in the formation of sub-haploid gametes and halting transmission. Our work identified an evolutionary divergent Arp2/3 protein complex in malaria parasites, offers novel insights into gametogenesis, and uncovers potential targets for transmission-blocking interventions.
HostingRepository	PRIDE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_05:16:42.488.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcin Luzarowski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Plasmodium berghei; NCBI TaxID: 5821;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-19 23:46:57	ID requested
⏵ 1	2025-04-22 05:16:43	announced

Dataset with its publication pending

submitter keyword: Arp2/3 complex, chromosome segregation,Malaria, Plasmodium

Marcin Luzarowski
contact affiliation	Core Facility for Mass Spectrometry and Proteomics, Center for Molecular Biology of Heidelberg University (ZMBH)
contact email	m.luzarowski@zmbh.uni-heidelberg.de
lab head
Marcin Luzarowski
contact affiliation	University of Heidelberg
contact email	m.luzarowski@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD060997

PRIDE project URI

• PRIDE
  1. PXD060997
     1. Label: PRIDE project
     2. Name: An atypical Arp2/3 complex is required for Plasmodium DNA segregation and transmission of malaria.