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PXD060997

PXD060997 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn atypical Arp2/3 complex is required for Plasmodium DNA segregation and transmission of malaria.
DescriptionPlasmodium parasites, the causative agents of malaria, undergo crucial developments within the mosquito vector, initiated by the formation of male and female gametes from gametocytes. Male gametogenesis involves three rapid rounds of endomitosis followed by a single round of DNA segregation and nuclear division during gamete budding. How the cell organises the segregation of eight genomes from a single octoploid nucleus into eight haploid gametes is currently unknown. Here, we discovered an atypical five-subunit Arp2/3 complex in Plasmodium important for correct DNA segregation during male gametogenesis. Unlike the canonical seven-subunit Arp2/3 complex found in other eukaryotes, Plasmodium Arp2/3 localizes to endomitotic spindles and interacts with a kinetochore-associated protein. Disruption of key Arp2/3 subunits or actin polymerization interferes with kinetochore-spindle association, resulting in the formation of sub-haploid gametes and halting transmission. Our work identified an evolutionary divergent Arp2/3 protein complex in malaria parasites, offers novel insights into gametogenesis, and uncovers potential targets for transmission-blocking interventions.
HostingRepositoryPRIDE
AnnounceDate2025-04-22
AnnouncementXMLSubmission_2025-04-22_05:16:42.488.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarcin Luzarowski
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Plasmodium berghei; NCBI TaxID: 5821;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-19 23:46:57ID requested
12025-04-22 05:16:43announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arp2/3 complex, chromosome segregation,Malaria, Plasmodium
Contact List
Marcin Luzarowski
contact affiliationCore Facility for Mass Spectrometry and Proteomics, Center for Molecular Biology of Heidelberg University (ZMBH)
contact emailm.luzarowski@zmbh.uni-heidelberg.de
lab head
Marcin Luzarowski
contact affiliationUniversity of Heidelberg
contact emailm.luzarowski@zmbh.uni-heidelberg.de
dataset submitter
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Dataset FTP location
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