PXD060976 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of the interactome of B2-SINE RNA transcripts |
Description | B2-SINEs are noncoding RNAs which are transcribed by RNA polymerase III (Pol III) from short interspersed nuclear elements (SINEs), which are high copy number transposable elements in the mouse genome. Unexpectedly have observed significant induction of non-coding RNAs from the B2-SINE subclass of repeat element RNAs in DRG neurons following sciatic nerve injury. We next sought to identify intracellular targets of GI-SINEs, by protein pull-down from sciatic nerve axoplasm using biotinylated B2-SINE RNA as bait. We generated two constructs from the B2 consensus sequence, comprising 5’ (1-75nt) and 3’ (75-166nt) sequences, both predicted to retain their respective structures, and used the constructs to pull down axoplasm proteins for mass spectrometry analysis. Further confirmatory experiments were performed using the mentioned -175nt sequence vs a control U1 SL3+4 RNA |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-05 |
AnnouncementXML | Submission_2025-05-05_12:18:38.388.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Juan Oses-Prieto |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-19 11:13:12 | ID requested | |
⏵ 1 | 2025-05-05 12:18:38 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: B2-SINE RNAs,RNA-Protein interactions, RNA regulators |
Contact List
Mike Fainzilber |
contact affiliation | Departments of Molecular Neuroscience and Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel |
contact email | Mike.Fainzilber@weizmann.ac.il |
lab head | |
Juan Oses-Prieto |
contact affiliation | UCSF |
contact email | joses@cgl.ucsf.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060976 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060976
- Label: PRIDE project
- Name: Analysis of the interactome of B2-SINE RNA transcripts