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PXD060976

PXD060976 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of the interactome of B2-SINE RNA transcripts
DescriptionB2-SINEs are noncoding RNAs which are transcribed by RNA polymerase III (Pol III) from short interspersed nuclear elements (SINEs), which are high copy number transposable elements in the mouse genome. Unexpectedly have observed significant induction of non-coding RNAs from the B2-SINE subclass of repeat element RNAs in DRG neurons following sciatic nerve injury. We next sought to identify intracellular targets of GI-SINEs, by protein pull-down from sciatic nerve axoplasm using biotinylated B2-SINE RNA as bait. We generated two constructs from the B2 consensus sequence, comprising 5’ (1-75nt) and 3’ (75-166nt) sequences, both predicted to retain their respective structures, and used the constructs to pull down axoplasm proteins for mass spectrometry analysis. Further confirmatory experiments were performed using the mentioned -175nt sequence vs a control U1 SL3+4 RNA
HostingRepositoryPRIDE
AnnounceDate2025-05-05
AnnouncementXMLSubmission_2025-05-05_12:18:38.388.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJuan Oses-Prieto
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-19 11:13:12ID requested
12025-05-05 12:18:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: B2-SINE RNAs,RNA-Protein interactions, RNA regulators
Contact List
Mike Fainzilber
contact affiliationDepartments of Molecular Neuroscience and Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel
contact emailMike.Fainzilber@weizmann.ac.il
lab head
Juan Oses-Prieto
contact affiliationUCSF
contact emailjoses@cgl.ucsf.edu
dataset submitter
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Dataset FTP location
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