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PXD060969

PXD060969 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification and validation of protective glycoproteins in Haemonchus contortus H11
DescriptionBarbervax is the first and only available vaccine to protect animals against Haemonchus contortus - one of the most pathogenic parasites of small ruminants. This vaccine contains a kind of native antigen called H11, a glycoprotein complex derived from integral gut of this parasite. Native H11 has been shown to induce high levels (72-95%) of protection, but single or two recombinant molecules of H11 are consistently unsuccessful. An increasing number of aminopeptidases related to H11 have been characterized in the past three decades, but little is known about which ones are the key contributors to protective immunity. Our recent work has revealed that the immunoprotective effect of H11 is primarily associated with its N-glycan moieties. To identify key immunoprotective glycoproteins derived from H11 antigen, we employed glycan-related protective IgG antibodies combined with LC-MS/MS analysis and identified five glycosylated H11 proteins: H11, H11-1, H11-2, H11-4, and H11-5. Subsequently, we utilized the baculovirus-insect cell expression system and successfully expressed four H11 recombinant proteins including rH11, rH11-1, rH11-2 and rH11-4, which demonstrated similar aminopeptidase activity and comparable high-mannose and di-fucosylated N-glycan structures to those found on native H11. Immunisation of goats with a cocktail of four rH11s induced a 66.29% reduction (p > 0.05) in total worm burden and cumulative faecal egg counts. High level of anti-rH11s IgG which could inhibit H. contortus intestinal aminopeptidase activity and larval development. Collectively, our study identified glycoprotein antigens from H11 and assessed their protective efficacy of a recombinant cocktail expressed in insect cells. This work will provide valuable insights into further development of recombinant vaccines against parasitic nematodes.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_06:56:05.308.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHui Liu
SpeciesList scientific name: Haemonchus contortus (Barber pole worm); NCBI TaxID: 6289;
ModificationListcomplex glycosylation
Instrument5800 TOF/TOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-19 09:38:24ID requested
12025-05-07 06:56:06announced
Publication List
Liu H, Zhang Y, Li J, Liu F, Ye L, Liu X, Wang C, Hu M, H11. Front Immunol, 16():1521022(2025) [pubmed]
10.3389/fimmu.2025.1521022;
Keyword List
submitter keyword: Haemonchus contortus, N-glycan, H11, immunoprotection, vaccine, glycoprotein, insect cells, recombinant antigen
Contact List
Chunqun Wang
contact affiliationHuazhong Agricultural University
contact emailwangchunqun@mail.hzau.edu.cn
lab head
Hui Liu
contact affiliationHuazhong Agricultural University
contact emailliuhui45@webmail.hzau.edu.cn
dataset submitter
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