PXD060969
PXD060969 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification and validation of protective glycoproteins in Haemonchus contortus H11 |
Description | Barbervax is the first and only available vaccine to protect animals against Haemonchus contortus - one of the most pathogenic parasites of small ruminants. This vaccine contains a kind of native antigen called H11, a glycoprotein complex derived from integral gut of this parasite. Native H11 has been shown to induce high levels (72-95%) of protection, but single or two recombinant molecules of H11 are consistently unsuccessful. An increasing number of aminopeptidases related to H11 have been characterized in the past three decades, but little is known about which ones are the key contributors to protective immunity. Our recent work has revealed that the immunoprotective effect of H11 is primarily associated with its N-glycan moieties. To identify key immunoprotective glycoproteins derived from H11 antigen, we employed glycan-related protective IgG antibodies combined with LC-MS/MS analysis and identified five glycosylated H11 proteins: H11, H11-1, H11-2, H11-4, and H11-5. Subsequently, we utilized the baculovirus-insect cell expression system and successfully expressed four H11 recombinant proteins including rH11, rH11-1, rH11-2 and rH11-4, which demonstrated similar aminopeptidase activity and comparable high-mannose and di-fucosylated N-glycan structures to those found on native H11. Immunisation of goats with a cocktail of four rH11s induced a 66.29% reduction (p > 0.05) in total worm burden and cumulative faecal egg counts. High level of anti-rH11s IgG which could inhibit H. contortus intestinal aminopeptidase activity and larval development. Collectively, our study identified glycoprotein antigens from H11 and assessed their protective efficacy of a recombinant cocktail expressed in insect cells. This work will provide valuable insights into further development of recombinant vaccines against parasitic nematodes. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_06:56:05.308.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hui Liu |
SpeciesList | scientific name: Haemonchus contortus (Barber pole worm); NCBI TaxID: 6289; |
ModificationList | complex glycosylation |
Instrument | 5800 TOF/TOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-02-19 09:38:24 | ID requested | |
⏵ 1 | 2025-05-07 06:56:06 | announced |
Publication List
Liu H, Zhang Y, Li J, Liu F, Ye L, Liu X, Wang C, Hu M, H11. Front Immunol, 16():1521022(2025) [pubmed] |
10.3389/fimmu.2025.1521022; |
Keyword List
submitter keyword: Haemonchus contortus, N-glycan, H11, immunoprotection, vaccine, glycoprotein, insect cells, recombinant antigen |
Contact List
Chunqun Wang | |
---|---|
contact affiliation | Huazhong Agricultural University |
contact email | wangchunqun@mail.hzau.edu.cn |
lab head | |
Hui Liu | |
contact affiliation | Huazhong Agricultural University |
contact email | liuhui45@webmail.hzau.edu.cn |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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