PXD060959

PXD060959 is an original dataset announced via ProteomeXchange.

Title	Bile effect on the profile of produced extracellular vesicles of Lactiplantibacillus plantarum.
Description	Bacterial extracellular vesicles (EVs), lipid bilayered particles ranging from 20 to 400 nm, are secreted by bacteria and carry cargo including proteins, lipids, and nucleic acids. EVs composition varies between Gram-positive and Gram-negative bacteria, and they contain pathogen recognition receptors that facilitate host interactions. This study utilized Lactobacillus plantarum as a model organism to investigate stress response mechanisms. Using size-exclusion chromatography (SEC), we isolated L. plantarum EVs (LpEVs) following MISEV guidelines. When bacteria were exposed to 0.25% bile, the resulting LpEVs increased in size and protein content, notably for proteins linked to bile metabolism. Our findings suggest that variability in LpEV profiles may act as an early stress response mechanism, providing insights into bacterial adaptability even prior to observable changes in the parent organism.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_18:18:40.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Václav Pustka
SpeciesList	scientific name: Lactobacillus plantarum; NCBI TaxID: NEWT:1590; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-19 06:18:59	ID requested
⏵ 1	2026-09-06 18:18:40	announced

10.20517/evcna.2026.26;

Razim A, Laimer-Digruber A, Schmid AM, Edelbacher TV, Paschall ME, Weinmayer T, Thaler M, Kebreab R, Skalska ME, Migda, ł P, Morandi M, Daniel C, Srutkova D, Schwarzer M, Hrdy J, Ehling-Schulz M, G, ó, rska S, Inic-Kanada A, Wiedermann U, Schabussova I, . Extracell Vesicles Circ Nucl Acids, 7(2):786-812(2026) [pubmed]

submitter keyword: stress, proteomics, bile salt hydrolase, bacteria

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Václav Pustka
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	vaclav.pustka@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD060959

PRIDE project URI

• PRIDE
  1. PXD060959
     1. Label: PRIDE project
     2. Name: Bile effect on the profile of produced extracellular vesicles of Lactiplantibacillus plantarum.