PXD060955 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A mechanism of YAP/TAZ transactivation and dual targeting for cancer therapy |
Description | Transcriptional coactivators of YAP/TAZ play key roles in cancers through transcriptional outputs. However, the mechanisms of their transactivation remain unclear, and effective and safe targeting solutions are lacking. Here, we discovered that YAP/ TAZ possess a hydrophobic transactivation domain (TAD). Knockout of TADs prevents tumor establishment due to growth defects and enhanced immune attack. TADs facilitate preinitiation complex (PIC) assembly by promoting TFIID recruitment in a TAF4-dependent manner and enhance RNA polymerase II (Pol II) elongation through MED15-dependent mediator complex recruitment. The bindings of TAD to the surface hydrophobic groove of MED15 induce its folding of the helix and hydrophobic interactions of TAD-MED15 boosts YAP-MED15 co-condensations thereby enhancing transcriptional hub formation and the expression of an oncogenic and immune suppressive transcriptional program. Synthesized small peptide TJ-M11 selectively disrupts the interactions of TADs with MED15 and TAF4, thereby suppressing tumor growth and sensitizing immunotherapy. This study indicates TADs of YAP/TAZ exhibit dual functions in PIC assembly and Pol II elongation, relying on hydrophobic interactions. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-02 |
AnnouncementXML | Submission_2025-04-01_19:37:51.434.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Man Yu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-19 05:35:31 | ID requested | |
⏵ 1 | 2025-04-01 19:37:51 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cancer therapy,YAP/TAZ,transactivation |
Contact List
Shuguo Sun |
contact affiliation | Huazhong University of Science and Technology |
contact email | shuguo@hust.edu.cn |
lab head | |
Man Yu |
contact affiliation | Huazhong University of Science and Techonology |
contact email | yuman@hust.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060955
- Label: PRIDE project
- Name: A mechanism of YAP/TAZ transactivation and dual targeting for cancer therapy