PXD060940

PXD060940 is an original dataset announced via ProteomeXchange.

Title	Cocktail Chemical Labeling for In-Depth Surfaceome Profiling
Description	We report a Ruthenium (Ru)-catalyzed cocktail labeling strategy that offers high catalytic efficiency and deeper proteome coverage through a straightforward “mix and lighten” workflow. Our approach uses a highly efficient transition metal complex (TMC)-based catalyst, along with two compatible biotin probes for biased substrates. Our results highlight the potential roles of many previously underestimated CSPs. We provide solid and detailed evidence supporting the efficacy of our method, demonstrating the advantages and uniqueness of Ru-based proximity labeling. This 'cocktail labeling' concept mitigates biases from different labeling methods, broadening substrate scopes and potentially inspiring advancements in other proximity labeling methods.
HostingRepository	iProX
AnnounceDate	2025-02-19
AnnouncementXML	Submission_2025-02-18_19:36:39.355.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yun Ge
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-18 19:36:19	ID requested
⏵ 1	2025-02-18 19:36:39	announced

Dataset with its publication pending

submitter keyword: Ruthenium, photocatalysis, cell surface proteome

Yun Ge
contact affiliation	Shenzhen Bay Laboratory
contact email	geyun@szbl.ac.cn
lab head
Yun Ge
contact affiliation	Shenzhen Bay Laboratory
contact email	geyun@szbl.ac.cn
dataset submitter

iProX dataset URI