PXD060879

PXD060879 is an original dataset announced via ProteomeXchange.

Title	Rat Tibialis Anterior PRT LCMSMS Soluble Fraction
Description	Resistance training (RT) promotes muscle protein accretion and myofiber hypertrophy, driven by dynamic processes of protein synthesis and degradation. While molecular studies have focused on acute signalling or long-term hypertrophy and strength gains, a critical gap remains in understanding the intermediate processes of muscle adaptation. Acute signalling does not always correlate directly with long-term outcomes, highlighting the need for a time-course analysis of protein abundance and turnover rates. To address this, we utilised deuterium oxide labelling and peptide mass spectrometry to quantify absolute protein content and synthesis rates in skeletal muscle. A daily programmed resistance training regimen was applied to the rat tibialis anterior (TA) via electrical stimulation of the left hind limb for 10, 20, and 30 days (5 sets of 10 repetitions daily). Muscle samples from stimulated (Stim) and contralateral control (Ctrl) limbs were analysed, quantifying 658 protein abundances and 215 protein synthesis rates.
HostingRepository	PRIDE
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-21_11:26:41.162.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060879
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jatin Burniston
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-17 07:54:30	ID requested
⏵ 1	2025-10-21 11:26:41	announced

10.1152/AJPCELL.00254.2025;

10.6019/PXD060879;

submitter keyword: Rat, Skeletal Muscle, LCMSMS

Jatin George Burniston
contact affiliation	Research institute for sport and exercise sciences, Liverpool John Moores University
contact email	j.burniston@ljmu.ac.uk
lab head
Jatin Burniston
contact affiliation	Liverpool John Moores University
contact email	j.burniston@ljmu.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060879
     1. Label: PRIDE project
     2. Name: Rat Tibialis Anterior PRT LCMSMS Soluble Fraction