PXD060866

PXD060866 is an original dataset announced via ProteomeXchange.

Title	Involvement of RNase J in CRISPR RNA maturation and degradosome formation in cyanobacteria
Description	Many bacteria and archaea use CRISPR-Cas systems, which provide RNA-based, adaptive, and inheritable immune defenses against invading viruses and other foreign genetic elements. The proper processing of CRISPR guide RNAs (crRNAs) is a crucial step in the maturation of the defense complexes and is frequently performed by specialized ribonucleases encoded by cas genes. However, some systems employ enzymes associated with degradosome or housekeeping functions, such as RNase III or the endoribonuclease RNase E. Here, the endo- and 5´-exoribonuclease RNase J was identified as additional enzyme involved in crRNA maturation, acting jointly with RNase E in the crRNA maturation of a type III-Bv CRISPR-Cas system, and possibly together with a further RNase. Co-IP experiments revealed a small set of proteins that were co-enriched with RNase J, among them PNPase. Despite a measured, strong 3’ exonucleolytic activity of the recombinant enzyme, PNPase was not confirmed to contribute to crRNA maturation. However, the co-IP results indicate that PNPase is a component of the cyanobacterial degradosome that can recruit either RNase E or RNase J, together with additional enriched proteins.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_17:27:52.680.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Friedel Drepper
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: NEWT:1148;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-17 04:16:32	ID requested
⏵ 1	2025-10-05 17:27:53	announced

Bilger R, Drepper F, Knapp B, Berndt T, Landerer H, Putzer H, Huesgen PF, Hess WR, sp. PCC 6803. Microlife, 6():uqaf022(2025) [pubmed]

10.1093/femsml/uqaf022;

submitter keyword: cyanobacteria, degradosome, ribonuclease J, RNA structure, RNA:RNA interaction, gene expression regulation,CRISPR-Cas systems

Pitter Huesgen
contact affiliation	Faculty of Biology, University of Freiburg, Freiburg, Germany
contact email	pitter.huesgen@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliation	University of Freiburg
contact email	f.drepper@biologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD060866

PRIDE project URI

• PRIDE
  1. PXD060866
     1. Label: PRIDE project
     2. Name: Involvement of RNase J in CRISPR RNA maturation and degradosome formation in cyanobacteria