PXD060865

PXD060865 is an original dataset announced via ProteomeXchange.

Title	Effect of mosquito saliva from distinct species on human dermal endothelial cell function in vitro and West Nile virus pathogenesis in vivo
Description	During probing and feeding, an infected mosquito injects both virus and saliva into the host skin. The presence of mosquito saliva in the skin increases arbovirus pathogenesis in the bitten host, however the exact mechanism behind this remains to be determined. It is hypothesized that disease enhancement is dependent on the function of the dermal endothelium, where an increased permeability aids in the influx of virus-susceptible cells to the bite site and therefore more cells for the virus to replicate in. Here, we investigate and compare the effects of saliva from Culex and Aedes species on the human dermal endothelial cell function in vitro. Furthermore, we investigate the effect of Culex saliva on West Nile virus (WNV) pathogenesis in a mouse model. We found that salivary gland extract from anthropophilic mosquito species (Aedes and Cx. pipiens molestus) induce permeability of the human dermal endothelium, while an ornithophilic mosquito species (Cx. pip. pipiens) does not. We identified that this effect is due to the presence of protease(s) in Cx. pipiens molestus saliva. In addition, we show that the presence of Cx. saliva at the WNV inoculation site in vivo leads to more consistent weight loss, increased permeability in the inoculation site, and increased mortality compared to inoculation of WNV alone. Moving forward, identification and characterization of novel salivary proteins from similar but genetically distinct mosquito species will advance the development of intervention methods to combat potential transmission risks and disease severity of emerging mosquito-borne pathogens.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:06:16.622.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeroen Demmers
SpeciesList	scientific name: West Nile virus; NCBI TaxID: NCBITaxon:11082; scientific name: Culex pipiens molestus; NCBI TaxID: 233155;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-17 02:19:48	ID requested
⏵ 1	2025-08-03 16:06:17	announced

Visser I, Vaes V, van Run P, Marshall EM, Vermaat L, Linthout C, Dekkers DHW, Demmers JAA, Koopmans MPG, Koenraadt CJM, Rissmann M, Rockx B, . Emerg Microbes Infect, 14(1):2502006(2025) [pubmed]

10.1080/22221751.2025.2502006;

submitter keyword: West Nile virus

arbovirus

mosquito saliva

pathogenesis

transmission

Culex pipiens

vascular leakage

Jeroen Demmers
contact affiliation	Proteomics Center, Erasmus MC
contact email	j.demmers@erasmusmc.nl
lab head
Jeroen Demmers
contact affiliation	Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands
contact email	j.demmers@erasmusmc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060865
     1. Label: PRIDE project
     2. Name: Effect of mosquito saliva from distinct species on human dermal endothelial cell function in vitro and West Nile virus pathogenesis in vivo