PXD060864

PXD060864 is an original dataset announced via ProteomeXchange.

Title	Hybrid hydrogel-extracellular matrix scaffolds identify distinct biochemical and mechanical signatures of cardiac ageing
Description	Extracellular matrix remodeling of cardiac tissue is a key contributor to age-related cardiovascular disease and dysfunction. Such remodeling is multifaceted including changes to biochemical composition, architecture, and mechanics, clouding our understanding of how and which extracellular matrix properties contribute to a dysfunctional state. Here we describe a decellularized extracellular matrix-synthetic hydrogel hybrid that independently presents two distinct matrix properties, ligand presentation and stiffness, to cultured cells in vitro, allowing for the identification of their specific roles in cardiac aging. The hybrid scaffold maintains native matrix composition and organization of young or aged murine cardiac tissue, while its mechanical properties can be independently tuned to mimic young or aged tissue stiffness. Seeding these scaffolds with murine primary cardiac fibroblasts, we identify distinct age- and matrix-dependent mechanisms of cardiac fibroblast activation, matrix remodeling, and senescence. Importantly, we show that ligand presentation of young extracellular matrix can outweigh profibrotic stiffness cues typically present in aged extracellular matrix in maintaining or driving cardiac fibroblast quiescence. Ultimately, these tunable scaffolds can enable the discovery of specific extracellular targets to prevent aging dysfunction and promote rejuvenation.
HostingRepository	PRIDE
AnnounceDate	2025-06-07
AnnouncementXML	Submission_2025-06-07_06:51:49.756.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Avery Sun
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-17 00:51:35	ID requested
⏵ 1	2025-06-07 06:51:50	announced

10.1038/S41563-025-02234-6;

submitter keyword: cardiac aging, mechanobiology, extracellular matrix

Jennifer Lauren
contact affiliation	Mechanobiology Institute, National University of Singapore
contact email	biejly@nus.edu.sg
lab head
Avery Sun
contact affiliation	National University of Singapore
contact email	sunrui99@u.nus.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD060864

PRIDE project URI

• PRIDE
  1. PXD060864
     1. Label: PRIDE project
     2. Name: Hybrid hydrogel-extracellular matrix scaffolds identify distinct biochemical and mechanical signatures of cardiac ageing