PXD060849

PXD060849 is an original dataset announced via ProteomeXchange.

Title	HDX-MS analysis of a light-oxygen-voltage sensor-containing histidine kinase
Description	The general stress response (GSR) protects bacteria from a wide range of stressors. In Alphaproteobacteria, GSR activation is coordinated by HWE/HisKA2 family histidine kinases (HKs), which can exhibit non-canonical structure and function. For example, while most light-oxygen-voltage sensor-containing HKs are light activated dimers, the Rubellimicrobium thermophilum RT-HK has inverted “dark on, light off” signaling logic with a tunable monomer/dimer equilibrium. Here, we further investigate these atypical behaviors of RT-HK and characterize its downstream signaling network. Using hydrogen-deuterium exchange mass spectrometry, we find that RT-HK uses a signal transduction mechanism similar to light-activated systems, despite its inverted logic.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-15_16:25:30.350.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rinat Abzalimov
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-15 22:11:30	ID requested
⏵ 1	2025-06-15 16:25:31	announced

10.1016/j.jbc.2025.108534;

Swingle D, Epstein L, Aymon R, Isiorho EA, Abzalimov RR, Favaro DC, Gardner KH, Variations in kinase and effector signaling logic in a bacterial two component signaling network. J Biol Chem, 301(6):108534(2025) [pubmed]

submitter keyword: HDX-MS, histidine kinase

Kevin H. Gardner
contact affiliation	Structural Biology Initiative, CUNY Advanced Science Research Center, New York, NY 10031
contact email	kgardner@gc.cuny.edu
lab head
Rinat Abzalimov
contact affiliation	ASRC CUNY
contact email	rabzalimov@gc.cuny.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD060849

PRIDE project URI

• PRIDE
  1. PXD060849
     1. Label: PRIDE project
     2. Name: HDX-MS analysis of a light-oxygen-voltage sensor-containing histidine kinase