PXD060837

PXD060837 is an original dataset announced via ProteomeXchange.

Title	High Enzyme Promiscuity in Lignin Degradation Mechanisms in Rhodopseudomonas palustris CGA009
Description	Lignin is a universal waste product of the agricultural industry and is currently seen as a potential feedstock for more sustainable manufacturing. While it is the second most abundant biopolymer in the world, most of it is currently burned as it is a very recalcitrant material. Many recent studies, however, have demonstrated the viability of biocatalysis to improve the value of this feedstock and convert it into more useful chemicals, such as polyhydroxybutyrate, and clean fuels like hydrogen and n-butanol. Rhodopseudomonas palustris is a gram-negative bacterium which demonstrates a plethora of desirable metabolic capabilities, including aromatic catabolism useful for lignin degradation. This study uses a multi-omics approach, including the first usage of CRISPRi in R. palustris, to investigate the lignin consumption mechanisms of R. palustris, the essentiality of redox homeostasis to lignin consumption, elucidate a potential lignin catabolic superpathway, and enable more economically viable sustainable lignin valorization processes.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_18:22:57.868.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophe Alvarez
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Rhodopseudomonas palustris; NCBI TaxID: 1076;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-14 12:59:53	ID requested
⏵ 1	2025-08-24 18:22:58	announced

10.1128/aem.00573-25;

Kathol M, Chowdhury NB, Immethun C, Alsiyabi A, Morris D, Naldrett MJ, Saha R, CGA009. Appl Environ Microbiol, 91(8):e0057325(2025) [pubmed]

submitter keyword: proteomics,Rhodopseudomonas palustris, label-free

Sophie Alvarez
contact affiliation	Proteomics and Metabolomics Facility, University of Nebraska- Lincoln
contact email	salvarez@unl.edu
lab head
Sophe Alvarez
contact affiliation	University of Nebraska - Lincoln
contact email	salvarez@unl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060837
     1. Label: PRIDE project
     2. Name: High Enzyme Promiscuity in Lignin Degradation Mechanisms in Rhodopseudomonas palustris CGA009