PXD060821

PXD060821 is an original dataset announced via ProteomeXchange.

Title	Histidine methylation of MYLK2 and C3H zinc fingers by CARNMT1
Description	We here describe our independent uncovering of CARNMT1 as a protein histidine methyltransferase (HMT), and our extensive efforts on defining its substrate specificity and methylation targets. We found that recombinant CARNMT1 methylated MYLK2-derived peptide containing His-148 in vitro. The recombinant CARNMT1 methylated also several proteins in extracts from CARNMT1 KO cells, and, using protein mass spectrometry, we identified several fully methylated CARNMT1 targets, all of which were C3H zinc finger (C3H ZnF) proteins. These included the previously identified U2AF1, ZC3H15 and ZC3H18, but also the novel substrates RBM22, PPP1R10, PRR3 and RNF113A. We also identified several of the homologous methylation events in Caenorhabditis elegans and showed that they could be introduced by the nematode CARNMT in vitro.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_16:35:47.961.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060821
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Manuel Ramirez
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-14 05:43:34	ID requested
⏵ 1	2025-07-27 16:35:48	announced

Ma, ł, ecki JM, Weirich S, Ramirez-Garrastacho M, Hagen L, Al-Egly J, Anonsen JH, Schroer L, Herrera MC, Davydova E, Slupphaug G, Jeltsch A, Falnes P Ø, Identification of substrates and sequence requirements for CARNMT1-mediated histidine methylation of C3H zinc fingers. J Biol Chem, 301(7):110335(2025) [pubmed]

10.6019/PXD060821;

10.1016/j.jbc.2025.110335;

submitter keyword: C3H zinc finger, 1MH, 1-methylhistidine, MYLK2, spliceosome, histidine methyltransferase, RNF113A,Protein methylation, CARNMT1, U2AF1

Jedrzej Malecki
contact affiliation	ection for Biochemistry and Molecular Biology, Department of Biosciences, University of Oslo
contact email	j.m.malecki@ibv.uio.no
lab head
Manuel Ramirez
contact affiliation	University of Oslo
contact email	manuelra@uio.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060821
     1. Label: PRIDE project
     2. Name: Histidine methylation of MYLK2 and C3H zinc fingers by CARNMT1