PXD060814

PXD060814 is an original dataset announced via ProteomeXchange.

Title	Ldm1 interaction proteomics of Ldm1-GFP cells
Description	Organelle motility enables strategic cellular reorganizations. In yeast, this process depends on the actin cytoskeleton, type V myosin motor proteins, and organelle-specific myosin-adaptor proteins. While the myosin-adaptors for most organelles are known, the factors that couple myosin to lipid droplets (LDs), the cellular lipid storage organelles, remained enigmatic. Using genome-wide screening, we identified Ldm1 (Lipid Droplet Motility 1/Yer085c) as a myosin-adaptor. Ldm1 binds to the globular tail domain of the myosin Myo2 and to the LD surface protein Ldo16 to enable actin-dependent LD motility. Ldo16 has additional roles in LD contact sites to the vacuole and the ER, suggesting a coordination of LD motility and organelle tethering. Ldm1 has a second role in mitochondrial transport and elevated Ldm1 levels rescue defects of the mitochondrial Myo2-adaptors Mmr1/Ypt11. Our work identifies the molecular machinery for LD motility and contributes to a comprehensive understanding of actin/myosin-based cellular reorganization.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-23_02:48:05.854.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060814
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bianca Esch
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-14 04:30:30	ID requested
⏵ 1	2026-02-23 02:48:06	announced

10.6019/PXD060814;

10.1016/J.CELREP.2025.116475;

submitter keyword: QExactivePlus, affinity purification, S. cerevisiae,LC-MSMS

Florian Fröhlich
contact affiliation	Bioanalytical Chemistry Section, Department of Biology/Chemistry, Osnabrück University, Germany
contact email	florian.froehlich@uni-osnabrueck.de
lab head
Bianca Esch
contact affiliation	Osnabrück University
contact email	bianca.esch@uos.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060814
     1. Label: PRIDE project
     2. Name: Ldm1 interaction proteomics of Ldm1-GFP cells