PXD060805
PXD060805 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative proteomics of EamB transporter in Aeromonas hydrophila biofilm formation |
| Description | EamB is an inner membrane transporter protein implicated in cysteine and O-acetylserine efflux, as well as in regulating protein synthesis. In this study, we investigated the role of the EamB transporter in biofilm formation of the fish pathogen Aeromonas hydrophila. Deletion of the eamB gene significantly impaired bacterial biofilm formation. Quantitative proteomic analysis identified 616 differentially expressed proteins between the ΔeamB mutant and wild-type (WT) strains, with 308 proteins downregulated and 308 proteins upregulated. Bioinformatic analysis revealed that EamB is involved in critical biological processes, including flagellar assembly, amino acid metabolism, and fatty acid degradation. Microbiological assays demonstrated that exogenous lysine enhanced biofilm formation in the ΔeamB strain but suppressed it in the WT strain, while β-alanine inhibited biofilm formation in the WT strain but had no significant effect on the ΔeamB mutant. |
| HostingRepository | iProX |
| AnnounceDate | 2025-02-14 |
| AnnouncementXML | Submission_2025-02-14_00:30:40.441.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Qiaozhen Xu |
| SpeciesList | scientific name: Aeromonas hydrophila subsp. hydrophila ATCC 7966; NCBI TaxID: 380703; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-02-14 00:30:23 | ID requested | |
| ⏵ 1 | 2025-02-14 00:30:40 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: EamB, amino acid transport, Biofilm formation, Proteomics, Aeromonas hydrophila |
Contact List
| Xiangmin Lin | |
|---|---|
| contact affiliation | Fujian Agriculture and Forestry University |
| contact email | 313461278@qq.com |
| lab head | |
| Qiaozhen Xu | |
| contact affiliation | Fujian Agriculture and Forestry University |
| contact email | 3312159383@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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