PXD060785

PXD060785 is an original dataset announced via ProteomeXchange.

Title	Structural Dynamic Impacts of Mosaic SARS-CoV-2 Spike Heterotrimer
Description	Polyvalent vaccines are being widely investigated for their broadening immune responses against rapidly mutating pathogens. In response to the emergence of highly infectious SARS-CoV-2 variants of concern, bivalent mRNA vaccines, which encode the original Hu-1 variant and Omicron variant, have demonstrated a novel approach to achieving polyvalency. However, the structural basis for immunogen display in mRNA vaccines has been less studied: specifically, whether protomers from different SARS-CoV-2 variants can co-assemble into mosaic heterotrimer and how this might affect trimer stability, dynamics and antigenicity. To investigate this, we successfully purified mosaic spike assemblies from co-expression of genetically close strains: Omicron BA.2 and XBB (OX), and distant strains: Omicron BA.2 and original Hu-1 G614 (OG). The trimer stability and integrity are well maintained in the mosaic spike trimers. Hydrogen/deuterium-exchange mass spectrometry (HDX-MS) and biolayer-interferometry were used to investigate the mosaic spike dynamics and impacts on antigenicity and receptor binding. These are important issues to investigate as numerous vaccine studies employing gene delivery such as with the mRNA-lipid nanoparticle modality that are pursuing pan-coronavirus or universal influenza vaccines rely upon co-administration of panels of antigen variants. The study provides valuable information to help understand the impact of co-transfection and co-expression on the combinatorial antigens that are produced in these scenarios.
HostingRepository	PRIDE
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-16_23:08:34.842.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chengbo Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-13 12:20:11	ID requested
⏵ 1	2025-09-16 23:08:35	announced

10.1371/JOURNAL.PPAT.1013143;

submitter keyword: Spike glycoprotein,HDX-MS, SARS-CoV-2, Antigen display

Kelly Lee
contact affiliation	Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA
contact email	kklee@uw.edu
lab head
Chengbo Chen
contact affiliation	University of Washington
contact email	cchen103@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD060785

PRIDE project URI

• PRIDE
  1. PXD060785
     1. Label: PRIDE project
     2. Name: Structural Dynamic Impacts of Mosaic SARS-CoV-2 Spike Heterotrimer