PXD060769

PXD060769 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	VRN1-associated proteins in Arabidopsis thaliana plants during cold exposure
Description	The epigenetic silencing of the Arabidopsis floral repressor gene FLOWERING LOCUS C (FLC) is induced by a prolonged period of cold and promotes the developmental transition to flowering post-cold. FLC silencing requires the function of VRN1, a non-sequence-specific DNA binding protein. Here, we used Arabidopsis seedlings subjected to cold, carrying a FLAG-tagged VRN1 under its endogenous promoter in the vrn1FRI mutant background. We performed FLAG immunoprecipitation followed by mass spectrometry (IP-MS) on crosslinked tissue. The proteomics analysis revealed several proteins that support the role of VRN1 as a chromatin regulator. The identified proteins associated with VRN1 in vivo also support its contribution to gene silencing.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_09:00:23.277.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060769
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-13 05:40:44	ID requested
⏵ 1	2025-05-07 09:00:23	announced

Publication List

10.6019/PXD060769;

10.6019/PXD060769;

Keyword List

submitter keyword: Arabidopsis,Chromatin, epigenetic silencing, flowering

submitter keyword: Arabidopsis,Chromatin, epigenetic silencing, flowering

Contact List

Miguel Montez
contact affiliation	John Innes Centre Norwich Research Park Norwich NR4 7UH, UK
contact email	miguel.montez@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060769

PRIDE project URI

Repository Record List

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