PXD060762

PXD060762 is an original dataset announced via ProteomeXchange.

Title	Discovery from in-depth molecular analyses of giant viruses: Haptophyte-infecting viruses change the genome condensing proteins of dinoflagellates
Description	Giant viruses are extraordinary members of the virosphere due to their structural complexity and high diversity in gene content. Haptophytes are ecologically important primary producers in the ocean, and all known viruses that infect haptophytes are giant viruses. Our in-depth electron microscopic, phylogenomic and virion proteomic analyses of two haptophyte-infecting giant viruses, Haptolina ericina virus RF02 (HeV RF02) and Prymnesium kappa virus RF02 (PkV RF02), unravel their large capacity for host manipulation and arsenals that functions during the infection cycle from virus entry to release. The virus infection induces significant morphological changes of host cell that are manipulated to build a virus proliferation factory. Both viruses’ genomes encode a putative nucleoprotein (dinoflagellate/viral nucleoprotein; DVNP), which was also found in the virion proteome of PkV RF02. Phylogenetic analysis suggests that DVNPs are widespread in marine giant metaviromes. Furthermore, the analysis shows that the dinoflagellate homologues were possibly acquired from viruses of the order Imitervirales.
HostingRepository	PRIDE
AnnounceDate	2025-02-21
AnnouncementXML	Submission_2025-02-21_02:11:27.473.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060762
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Prymnesium kappa virus; NCBI TaxID: 1499987;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-13 03:20:54	ID requested
⏵ 1	2025-02-21 02:11:27	announced

10.6019/PXD060762;

submitter keyword: Haptolina ericina virus,Giant viruses, Haptophytes, Prymnesium kappa virus

Carmen San Martín
contact affiliation	Centro Nacional de Biotecnología (Macromolecular Structures
contact email	carmen@cnb.csic.es
lab head
Sergio Ciordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD060762

PRIDE project URI

• PRIDE
  1. PXD060762
     1. Label: PRIDE project
     2. Name: Discovery from in-depth molecular analyses of giant viruses: Haptophyte-infecting viruses change the genome condensing proteins of dinoflagellates