PXD060730

PXD060730 is an original dataset announced via ProteomeXchange.

Title	Kinome profiling of Nf2-null murine Schwann cell line MS03 with defactinib and VS-4718
Description	Patients with neurofibromatosis type 2-related schwannomatosis (NF2-SWN) develop hallmark tumors on their vestibular nerves, leading to hearing loss and numerous associated morbidities. Currently, there are no targeted therapeutic options for treating these tumors. In prior work, our group had identified brigatinib as a potentially active drug in the treatment of murine models of NF2-SWN and meningioma, leading to a clinical trial in NF2-SWN patients testing brigatinib. Kinome profiling revealed that brigatinib resulted in the inhibition of focal adhesion kinase 1 (FAK1, encoded by the PTK2 gene). To test the contribution of FAK1, we generated a genetically engineered mouse model of NF2-SWN with Fak1/Ptk2 deletion in tumorigenic cells of origin. Genetic ablation of Fak1/Ptk2 preserved hearing in the mice and reduced tumorigenesis. Here we used an immortalized Nf1 null Schwann cell line, MS03, to perform kinome profiling using the FAK inhibitors defactinib and VS-4718. Kinase enrichment proteomic analysis was performed using MS03 cells treated for 24 h with DMSO control, 1 micromolar defactinib, or 1 micromolar VS-4718 to evaluate the effects on the functional kinome.
HostingRepository	PRIDE
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-17_10:57:43.014.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Angus
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-12 09:32:00	ID requested
⏵ 1	2025-09-17 10:57:43	announced

Dataset with its publication pending

submitter keyword: focal adhesion kinase inhibitor,Neurofibromatosis type 2-related schwannomatosis, multiplexed kinase inhibitor beads, kinase inhibitor

D. Wade Clapp, MD
contact affiliation	Department of Pediatrics Indiana University School of Medicine Riley Hospital for Children
contact email	dclapp@iu.edu
lab head
Steven Angus
contact affiliation	Indiana University School of Medicine
contact email	sangus@iu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060730
     1. Label: PRIDE project
     2. Name: Kinome profiling of Nf2-null murine Schwann cell line MS03 with defactinib and VS-4718