PXD060688 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DdiA, an XRE family transcriptional regulator, regulates a LexA-independent DNA damage response in Myxococcus xanthus |
Description | Repair of DNA damage is essential for genome integrity. DNA damage elicits a DNA damage response (DDR) that includes error-free and error-prone, i.e. mutagenic, repair. The SOS response is a widely conserved system in bacteria that regulates the DDR and depends on the recombinase RecA and the transcriptional repressor LexA. However, RecA/LexA-independent DDRs have been identified in several bacterial species. Here, using a whole-cell, label-free quantitative proteomics approach, we map the proteomic response in Myxococcus xanthus to mitomycin C treatment and the lack of LexA. In doing so, we confirm a LexA-independent DDR in M. xanthus. Using a candidate approach, we identify DdiA, a transcriptional regulator of the XRE family, and demonstrate that it regulates a subset of the LexA-independent DDR genes. Further, we show that ddiA expression is activated heterogeneously in a subpopulation of cells in the absence of exogenous genotoxic stress and is reversibly activated population-wide in response to such stress. We show that DdiA, indirectly or directly, activates the expression of dnaE2, which encodes the DnaE2 error-prone DNA polymerase, and inhibits the expression of recX, which encodes RecX, a negative regulator of RecA. Accordingly, the ΔddiA mutant has a lower mutation frequency than the wild-type but also a fitness defect, suggesting that DdiA mediates a trade-off between fitness and mutagenesis. We speculate that the DdiA-dependent response is tailored to counter replication stress, thereby preventing the induction of the complete RecA/LexA-dependent DDR in the absence of exogenous genotoxic stress. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-12 |
AnnouncementXML | Submission_2025-02-12_00:43:50.671.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Timo Glatter |
SpeciesList | scientific name: Myxococcus xanthus DK 1622; NCBI TaxID: 246197; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-11 08:46:56 | ID requested | |
⏵ 1 | 2025-02-12 00:43:51 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Myxococcus xanthus ,DNA damage |
Contact List
Timo Glatter |
contact affiliation | Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany |
contact email | timo.glatter@mpi-marburg.mpg.de |
lab head | |
Timo Glatter |
contact affiliation | Max Planck Institute for Terrestrial Microbiology
Marburg |
contact email | timo.glatter@mpi-marburg.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060688
- Label: PRIDE project
- Name: DdiA, an XRE family transcriptional regulator, regulates a LexA-independent DNA damage response in Myxococcus xanthus