PXD060669

PXD060669 is an original dataset announced via ProteomeXchange.

Title	The discovery and molecular mechanism of the subversion of human E3 ligase RNF213 by the Shigella effector IpaH1.4
Description	Ubiquitination plays vital roles in modulating pathogen-host cell interactions. RNF213, a unique E3 ligase, can catalyze the ubiquitination of lipopolysaccharide (LPS) and is crucial for antibacterial immunity in mammals. Shigella flexneri, an LPS-containing pathogenic bacterium, has developed mechanisms to evade host antibacterial defenses during infection. However, the precise strategies by which Shigella flexneri circumvents RNF213-mediated antibacterial immunity remain poorly understood. Here, through comprehensive biochemical, structural and cellular analyses, we reveal that the E3 effector IpaH1.4 of Shigella flexneri can directly target human RNF213 via a specific interaction between the IpaH1.4 LRR domain and the RING domain of RNF213, and mediate the ubiquitination and proteasomal degradation of RNF213 in vitro and in cells. Furthermore, we determine the cryo-EM structure of human RNF213 and the crystal structure of the IpaH1.4 LRR/RNF213 RING complex, elucidating the molecular mechanism underlying the specific recognition of RNF213 by IpaH1.4. Finally, our cell-based function asaays demonstrate that the targeting of host RNF213 by IpaH1.4 promotes Shigella flexneri proliferation within infected cells. In summary, our work uncovers a novel strategy employed by Shigella flexneri to subvert the key host immune factor RNF213, thereby facilitating bacterial proliferation during invasion.
HostingRepository	iProX
AnnounceDate	2025-02-11
AnnouncementXML	Submission_2025-02-10_21:38:43.641.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xindi Zhou
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-10 21:38:26	ID requested
⏵ 1	2025-02-10 21:38:44	announced

Dataset with its publication pending

submitter keyword: shigella,IpaH1.4,LC-MS/MS

Lifeng Pan
contact affiliation	Shanghai Institute of Organic Chemistry
contact email	panlf@sioc.ac.cn
lab head
Xindi Zhou
contact affiliation	Shanghai Institute of Organic Chemistry
contact email	zhouxd@sioc.ac.cn
dataset submitter

iProX dataset URI