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PXD060654

PXD060654 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArabidopsis leaf apoplast proteomes under flg22 induced pattern-triggered immunity
DescriptionThe apoplast is a critical interface in plant-pathogen interactions particularly in the context of pattern-triggered immunity (PTI), which is initiated by recognition of microbe-associated molecular patterns (PAMPs). Our study characterizes the proteomic profile of the Arabidopsis apoplast during PTI induced by flg22, a 22 amino acid bacterial flagellin epitope, to elucidate the output of PTI. Apoplastic washing fluid (AWF) was extracted with minimal cytoplasmic contamination for LC-MS/MS analysis. We observed consistent identification of PTI enriched and depleted peptides across replicates with limited correlation between total protein abundance and transcript abundance. We observed topological bias in peptide recovery of receptor-like kinases with peptides predominantly recovered from their ectodomains. Notably, tetraspanin 8, an exosome marker, was enriched in PTI samples. We additionally confirmed increased concentrations of exosomes during PTI. This study enhances our understanding of the proteomic changes in the apoplast during plant immune responses and lays the groundwork for future investigations into the molecular mechanisms of plant defense under recognition of pathogen patterns.
HostingRepositoryPRIDE
AnnounceDate2025-02-11
AnnouncementXMLSubmission_2025-02-11_00:41:59.763.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD060654
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHsiao-Chun Chen
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-10 14:52:57ID requested
12025-02-11 00:42:00announced
Publication List
10.6019/PXD060654;
Keyword List
submitter keyword: Tetraspanin, Extracellular vesicle,Proteomics, Pattern-triggered immunity, flg22,Apoplast
Contact List
Brian Howard
contact affiliationDepartment of Plant Pathology, University of Georgia, Athens, Georgia, USA
contact emailbkvitko@uga.edu
lab head
Hsiao-Chun Chen
contact affiliationUniversity of Georgia
contact emailhcchen@uga.edu
dataset submitter
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