PXD060640

PXD060640 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of Chlamydomonas PetD mutants
Description	The cytochrome b6f complex (b6f) links photosystem II and photosystem I in photosynthetic electron transfer and is distributed between appressed and non-appressed thylakoid membranes. It also activates the state-transition 7 kinase (STT7), which phosphorylates light-harvesting complex proteins, facilitating energy redistribution between photosystems to optimize their efficiency. To investigate the role of STT7-dependent phosphorylation at T4 in the N-terminal domain of the b6f subunit-IV (PetD), we generated several chloroplast mutants. The phosphomimic mutation PetD T4E blocks STT7 kinase activity since proteomics show the absence of STT7-dependent phosphorylation and the strain being locked in State 1, revealing a novel feedback regulation mechanism. The deletion of five N-terminal amino acids resulted in a comparable inhibition of STT7 activity; furthermore, the additional disruption of electron transfer underscores an additional crucial role of the PetD N-terminus in b6f function.
HostingRepository	PRIDE
AnnounceDate	2026-04-04
AnnouncementXML	Submission_2026-04-04_10:29:15.021.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Scholz
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: NEWT:3055;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-10 08:41:59	ID requested
⏵ 1	2026-04-04 10:29:15	announced

Dataset with its publication pending

submitter keyword: PetD, Chlamydomonas, phosphorylation, STT7

Michael Hippler
contact affiliation	WWU Münster
contact email	mhippler@uni-muenster.de
lab head
Martin Scholz
contact affiliation	IBBP Muenster
contact email	mscholz@uni-muenster.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060640
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis of Chlamydomonas PetD mutants