PXD060631

PXD060631 is an original dataset announced via ProteomeXchange.

Title	Protein co-immmunoprecipitation using antibodies specific for the antimicrobial peptide FsPDF2 from Fagus sylvatica
Description	To assess the specificity of the antibodies used in the quantitative sandwich ELISA, co-immunoprecipitation (CoIP) was performed. Proteins binding to these antibodies were detected using LC-MS/MS in DDA mode.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:12:06.355.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Kucklick
SpeciesList	scientific name: Fagus sylvatica; NCBI TaxID: NEWT:28930;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-10 08:10:31	ID requested
⏵ 1	2026-06-07 16:12:07	announced

10.1111/pbi.70431;

Heine PA, Nosenko T, Kistner S, Oliphant KD, Hanke-Uhe M, Shahid A, Hu B, Kucklick M, Lehmler N, Becker M, Goerke N, Korn J, Linke T, Meier D, Perl A, Polten S, Priess V, Sch, ä, ckermann D, Schubert M, Schumacher J, Winkler JB, Engelmann S, Rennenberg H, Schnitzler JP, D, ü, bel S, Hust M, H, ä, nsch R, Kaufholdt D, Phage Display Derived Antibodies Against Antimicrobial Peptide FsPDF2 Reveal Stress Response in European Beech. Plant Biotechnol J, 24(4):1950-1965(2026) [pubmed]

submitter keyword: Co- immunoprecipitation (CoIP), antimicrobial peptides,Fagus sylvatica

Susanne Engelmann
contact affiliation	CPRO, Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
contact email	susanne.engelmann@helmholtz-hzi.de
lab head
Martin Kucklick
contact affiliation	TU Braunschweig Institut für Mikrobiologie Spielmannstr. 7 Germany
contact email	m.kucklick@tu-braunschweig.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD060631

PRIDE project URI

• PRIDE
  1. PXD060631
     1. Label: PRIDE project
     2. Name: Protein co-immmunoprecipitation using antibodies specific for the antimicrobial peptide FsPDF2 from Fagus sylvatica