PXD060628

PXD060628 is an original dataset announced via ProteomeXchange.

Title	Multi-Omics Characterization of the Virulence Potential of Bacillus thuringiensis Subspecies using an in vitro intestinal epithelial model
Description	The soil bacterium Bacillus thuringiensis (Bt) can produce spores and crystals composed of toxins with insecticidal activity. Due to these properties, some Bt strains have been developed as biopesticides for pest control, with a high value to restrict the use of chemical insecticides. However, Bt strains belong to the Bacillus cereus (Bc) group, sharing some virulence genes encoding diarrheal enterotoxins like hemolysin BL (Hbl), non-hemolytic enterotoxin (Nhe) and cytotoxin K (Cyt-K2). These toxins are known to be involved in foodborne outbreaks (FBOs) caused by Bc sensu stricto contamination. For this reason, it has been suggested that Bt may also provoke FBOs and it is therefore crucial to evaluate their virulence potential and to characterize their effects on humans. For this purpose, differentiated human intestinal Caco-2 cells, able to mimic the human intestinal barrier, have been treated with serial dilutions of the culture supernatant of five different Bt strains used as biopesticides. No cytotoxicity was induced with all Bt supernatants after 24 h treatment in contrast to Bacillus cytotoxicus (Bcyt) supernatants. Interleukin 8 (IL-8) secretion increased sharply in both groups, suggesting possible concerns with chronic inflammation. To investigate the molecular effects of Bt supernatants on Caco-2 cells, untargeted proteomics and metabolomics were performed on both cell media and lysates with 3 non-cytotoxic concentrations. Proteomics and metabolomics highlighted common perturbations on oxidative phosphorylation (OXPHOS) and lipid metabolism for both Bt and Bcyt supernatants exposures. Proteins linked to acute inflammation decreased only with Bcyt exposure. Bt supernatants and not Bcyt supernatants caused a shedding of CD59 and CD55, inhibitors of the complement system, from the cell membrane into the culture medium. This showed that Bt supernatants can increase the vulnerability of intestinal cells to their own immune system. Proteomics also revealed the heterogeneity of toxin abundance in the supernatants of the different Bt subspecies, demonstrating the importance of taking subspecies into account when assessing the risks associated with their use as biopesticides.
HostingRepository	PRIDE
AnnounceDate	2026-07-02
AnnouncementXML	Submission_2026-07-02_09:55:46.281.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD060628
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thibaut LEGER
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-10 08:02:48	ID requested
⏵ 1	2026-07-02 09:55:47	announced

10.6019/PXD060628;

10.1016/j.scitotenv.2026.181742;

Alilat S, Lanceleur R, Bonis M, Pairaud S, De Almeida NP, Vial G, Sarda X, Firmesse O, Fessard V, Liuu S, L, é, ger T, Multi-omics characterisation of the virulence and inflammatory potential of Bacillus thuringiensis in an in vitro intestinal epithelial model. Sci Total Environ, 1029():181742(2026) [pubmed]

submitter keyword: Bacillus thuringiensis

Bacillus cytotoxicus

proteomics

metabolomics

enterotoxins

Caco-2

Thibaut Léger
contact affiliation	Anses Fougères
contact email	thibaut.leger@anses.fr
lab head
Thibaut LEGER
contact affiliation	French Agency for Food, Environmental and Occupational Health & Safety (ANSES)
contact email	thibaut.leger@anses.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD060628

PRIDE project URI

• PRIDE
  1. PXD060628
     1. Label: PRIDE project
     2. Name: Multi-Omics Characterization of the Virulence Potential of Bacillus thuringiensis Subspecies using an in vitro intestinal epithelial model