PXD060621

PXD060621 is an original dataset announced via ProteomeXchange.

Title	Solvent-based Shift Assay in Living Cells
Description	Biophysical proteomics assays allow for proteome-wide, label-free monitoring of ligand-induced changes in protein structure and stability, offering insights into protein-ligand interactions and modulation of biophysical properties of cellular proteins. They exploit the principle that compound-induced alterations in structure or stability of proteins can be detected through changes in their susceptibility to denaturation. Here we introduce SPICE (Solvent Proteome Profiling In Cells), which employs solvent-based denaturation of proteins under otherwise physiological conditions in intact cells. We characterized solvent-induced denaturation of proteins inside cells as distinct from that in cell extracts and validated SPICE by detecting known drug-target interactions for multiple compound classes. We showed that SPICE can be flexibly used in different formats, to resolve denaturation or concentration-response curves upon treatment of live cells. Our results indicate that SPICE, unlike experiments in cell extracts, also detects secondary compound-induced effects such as target profiles of drug metabolites, modulation of protein-protein interactions, and downstream signalling events.
HostingRepository	PRIDE
AnnounceDate	2025-11-20
AnnouncementXML	Submission_2025-11-20_14:56:28.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominik Steinbrunn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-10 04:32:03	ID requested
⏵ 1	2025-11-20 14:56:28	announced

10.1016/J.MCPRO.2025.101444;

submitter keyword: Label-free drug target identification, human, intracellular target engagement, QE-Plus

Hannes Hahne
contact affiliation	OmicScouts GmbH, Freising, Germany
contact email	hannes.hahne@omicscouts.com
lab head
Dominik Steinbrunn
contact affiliation	Chair of Organic Chemistry II, Technical University Munich OmicScouts GmbH, Freising
contact email	d.steinbrunn@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD060621

PRIDE project URI

• PRIDE
  1. PXD060621
     1. Label: PRIDE project
     2. Name: Solvent-based Shift Assay in Living Cells