PXD060598 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DDX3X SUMOylation Promotes Septic Inflammation by Enhancing NLRP3 Inflammasome Activation |
Description | This study investigates the role of DDX3X SUMOylation in the activation of the NLRP3 inflammasome during sepsis. We demonstrate that SUMOylation of DDX3X, regulated by the SUMO protease SENP1, is a crucial trigger for NLRP3 inflammasome activation. In endotoxemia and sepsis models, the absence of SENP1 in macrophages and mice led to increased NLRP3 inflammasome activation. Mechanistically, DDX3X SUMOylation promoted the interaction of NLRP3 at lysine 118, inhibiting K48-conjugated ubiquitination and subsequent degradation of NLRP3 by the deubiquitinase OTUD6A, resulting in enhanced oligomerization of NLRP3. In murine adoptive transfer experiments, macrophages expressing the SUMOylation-inhibiting mutation DDX3X-K118R suppressed NLRP3 inflammasome activation, providing protection against LPS-induced inflammatory lung injury. These findings suggest that SENP1-mediated DDX3X deSUMOylation regulates NLRP3 inflammasome activation, offering a potential therapeutic avenue to mitigate NLRP3-induced inflammation. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-06 |
AnnouncementXML | Submission_2025-03-06_06:00:26.546.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | xuedi zhang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-08 22:20:42 | ID requested | |
⏵ 1 | 2025-03-06 06:00:26 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
Contact List
xuedi zhang |
contact affiliation | Guangdong Medical university |
contact email | zhangxuedi1987@163.com |
lab head | |
xuedi zhang |
contact affiliation | Affiliated hospital of Guangdong Medical University |
contact email | zhangxuedi1987@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060598
- Label: PRIDE project
- Name: DDX3X SUMOylation Promotes Septic Inflammation by Enhancing NLRP3 Inflammasome Activation