PXD060598

PXD060598 is an original dataset announced via ProteomeXchange.

Title	DDX3X SUMOylation Promotes Septic Inflammation by Enhancing NLRP3 Inflammasome Activation
Description	This study investigates the role of DDX3X SUMOylation in the activation of the NLRP3 inflammasome during sepsis. We demonstrate that SUMOylation of DDX3X, regulated by the SUMO protease SENP1, is a crucial trigger for NLRP3 inflammasome activation. In endotoxemia and sepsis models, the absence of SENP1 in macrophages and mice led to increased NLRP3 inflammasome activation. Mechanistically, DDX3X SUMOylation promoted the interaction of NLRP3 at lysine 118, inhibiting K48-conjugated ubiquitination and subsequent degradation of NLRP3 by the deubiquitinase OTUD6A, resulting in enhanced oligomerization of NLRP3. In murine adoptive transfer experiments, macrophages expressing the SUMOylation-inhibiting mutation DDX3X-K118R suppressed NLRP3 inflammasome activation, providing protection against LPS-induced inflammatory lung injury. These findings suggest that SENP1-mediated DDX3X deSUMOylation regulates NLRP3 inflammasome activation, offering a potential therapeutic avenue to mitigate NLRP3-induced inflammation.
HostingRepository	PRIDE
AnnounceDate	2025-03-06
AnnouncementXML	Submission_2025-03-06_06:00:26.546.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xuedi zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-08 22:20:42	ID requested
⏵ 1	2025-03-06 06:00:26	announced

Dataset with its publication pending

submitter keyword: LC-MS

xuedi zhang
contact affiliation	Guangdong Medical university
contact email	zhangxuedi1987@163.com
lab head
xuedi zhang
contact affiliation	Affiliated hospital of Guangdong Medical University
contact email	zhangxuedi1987@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060598
     1. Label: PRIDE project
     2. Name: DDX3X SUMOylation Promotes Septic Inflammation by Enhancing NLRP3 Inflammasome Activation