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PXD060598

PXD060598 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDDX3X SUMOylation Promotes Septic Inflammation by Enhancing NLRP3 Inflammasome Activation
DescriptionThis study investigates the role of DDX3X SUMOylation in the activation of the NLRP3 inflammasome during sepsis. We demonstrate that SUMOylation of DDX3X, regulated by the SUMO protease SENP1, is a crucial trigger for NLRP3 inflammasome activation. In endotoxemia and sepsis models, the absence of SENP1 in macrophages and mice led to increased NLRP3 inflammasome activation. Mechanistically, DDX3X SUMOylation promoted the interaction of NLRP3 at lysine 118, inhibiting K48-conjugated ubiquitination and subsequent degradation of NLRP3 by the deubiquitinase OTUD6A, resulting in enhanced oligomerization of NLRP3. In murine adoptive transfer experiments, macrophages expressing the SUMOylation-inhibiting mutation DDX3X-K118R suppressed NLRP3 inflammasome activation, providing protection against LPS-induced inflammatory lung injury. These findings suggest that SENP1-mediated DDX3X deSUMOylation regulates NLRP3 inflammasome activation, offering a potential therapeutic avenue to mitigate NLRP3-induced inflammation.
HostingRepositoryPRIDE
AnnounceDate2025-03-06
AnnouncementXMLSubmission_2025-03-06_06:00:26.546.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterxuedi zhang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-08 22:20:42ID requested
12025-03-06 06:00:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC-MS
Contact List
xuedi zhang
contact affiliationGuangdong Medical university
contact emailzhangxuedi1987@163.com
lab head
xuedi zhang
contact affiliationAffiliated hospital of Guangdong Medical University
contact emailzhangxuedi1987@163.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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