PXD060591
PXD060591 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Canagliflozin ameliorates progestin resistance by suppressing RARβ/CRABP2 signaling in TRβ knockout endometrial cancer cells |
| Description | THRB-knockout RL95-2 (THRB(-/-)/RL95-2) cells were constructed to investigate progestin resistance mechanisms. Cell proliferation and apoptosis were assessed in RL95-2 and THRB(-/-)/RL95-2 cells treated with canagliflozin (CANA), medroxyprogesterone acetate (MPA), or their combination using CCK-8, EdU, and flow cytometry assays. In vivo, nude mouse xenograft models were used to evaluate CANA and MPA efficacy. Transcriptomic and proteomic analyses identified pathways associated with progestin resistance. Molecular dynamics simulations, western blotting, and immunohistochemistry identified CANA targets. Electrophoretic mobility shift assays and dual luciferase reporter assays evaluated the regulatory effects of TRβ, RARβ, and CRABP2. |
| HostingRepository | iProX |
| AnnounceDate | 2025-02-07 |
| AnnouncementXML | Submission_2025-08-10_21:24:51.436.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ye Yang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF SCP |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-02-07 19:05:21 | ID requested | |
| 1 | 2025-02-07 19:05:36 | announced | |
| 2 | 2025-07-16 00:41:46 | announced | 2025-07-16: Add info. |
| ⏵ 3 | 2025-08-10 21:24:52 | announced | 2025-07-16: Add info. 2025-08-11: Add publication. |
Publication List
| Yang Y, Zhou J, Lv Q, Ni Q, Hu B, Wang Y, Qu S, Li G, Yang W, Zhong R, Chen X, Zhu Y, /CRABP2 signaling in THRB knockout endometrial cancer cells. Front Pharmacol, 16():1573032(2025) [pubmed] |
Keyword List
| submitter keyword: endometrial cancer, thyroid hormone receptor β, canagliflozin, medroxyprogesterone acetate, progestin resistance, retinoic acid receptor β |
Contact List
| Yan Zhu | |
|---|---|
| contact affiliation | Fudan University |
| contact email | zhuyan@sippr.org |
| lab head | |
| Ye Yang | |
| contact affiliation | Fudan University |
| contact email | Pharmacy_yeyang@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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