PXD060590
PXD060590 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tyrosine/Lysine-Targeting Urazole Cross-Linkers for Protein Structural Analysis |
Description | Chemical cross-linking mass spectrometry (XL-MS) is essential for studying protein structures and interactions. While lysine-targeted cross-linkers dominate current applications, this study introduces two urazole-derived MS-cleavable cross-linkers for tyrosine and lysine residues. A homobifunctional cross-linker (SBT) targets tyrosine via electrochemical click chemistry, while a heterobifunctional cross-linker (SCT) binds both lysine and tyrosine. Their efficiency was validated through reactions with peptides, proteins, and complexes. Additionally, AlphaFold 3-predicted BSA dimer structures were compared with crystallographic data, revealing improved structural coverage. These cross-linkers expand the XL-MS toolkit, enhancing protein structural analysis accuracy and scope. |
HostingRepository | iProX |
AnnounceDate | 2025-02-07 |
AnnouncementXML | Submission_2025-02-07_19:03:54.665.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhonglin Wei |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-02-07 19:03:37 | ID requested | |
⏵ 1 | 2025-02-07 19:03:55 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cross-linking mass spectrometry, Electrochemical click reaction, Tyrosine residue reactive |
Contact List
Zhonglin Wei | |
---|---|
contact affiliation | Jilin University |
contact email | zlwei@jlu.edu.cn |
lab head | |
Zhonglin Wei | |
contact affiliation | Jilin University |
contact email | zlwei@jlu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |