PXD060583

PXD060583 is an original dataset announced via ProteomeXchange.

Title	Versican regulates Collagen fibrillation and Tenascin-C expression, suppressing podosome formation and pulmonary fibrosis
Description	The activation and accumulation of lung fibroblasts, leading to aberrant deposition of extracellular matrix (ECM) components, is a pathogenic hallmark of Idiopathic Pulmonary Fibrosis (IPF), a lethal and currently incurable disease. ECM deposition perpetuates fibroblast activation and stimulates ECM invasion via the formation of podosomes, an inherent property of IPF fibroblasts that is well amenable to pharmaceutical targeting. Proteoglycans (PGs), a significant component of the interstitial ECM, fine-tune the overall tissue architecture determined by fibrous proteins and regulate several ECM-controlled signalling pathways. In this report, increased expression of Versican (VCAN), a multifunctional PG, was detected in mouse and human pulmonary fibrosis, predominantly in monocytic cells and fibroblasts. Genetic downregulation of Vcan expression in mice modulated pulmonary ECM composition and organisation, promoted collagen expression and lung stiffness, exacerbating modelled pulmonary fibrosis and worsening respiratory functions. The decrease of Vcan levels in the ECM resulted in longer, thicker and tangled collagen fibrils, altered PG distribution, and increased levels of Tenascin-C (TNC), an ECM glycoprotein, shown here as a potent podosome inducer promoting ECM invasion. Thus, Vcan expression from fibroblasts is established as an autologous fibrotic brake, and TNC is suggested as a promising therapeutic target in IPF.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_17:45:30.952.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-07 04:23:02	ID requested
⏵ 1	2026-02-22 17:45:31	announced

Kanellopoulou P, Barbayianni I, Fanidis D, Samiotaki M, Katsiouli E, Nastos D, Smyrniotis S, Shira M, Galaris A, Rinotas V, Grammenoudi S, Magkrioti C, Tomos I, Martinez Blanco A, Tremi I, Vamvakaris I, Gavara N, Havaki S, Gorgoulis V, Watanabe H, Aidinis V, Versican expression from lung fibroblasts suppresses pulmonary fibrosis. Nat Commun, 17(1):1676(2026) [pubmed]

10.1038/s41467-026-68377-5;

submitter keyword: pulmonary fibrosis

extracellular matrix (ECM)

lung fibroblasts

podosomes

ECM invasion

Versican (VCAN)

Hyaluronan (HA)

collagen (COL)

Tenascin-c (TNC)

Vassilis Aidinis
contact affiliation	Institute for Fundamental Biomedical Research, Biomedical Sciences Research Center AlexanderFleming, Athens/Greece.
contact email	V.Aidinis@fleming.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD060583

PRIDE project URI

• PRIDE
  1. PXD060583
     1. Label: PRIDE project
     2. Name: Versican regulates Collagen fibrillation and Tenascin-C expression, suppressing podosome formation and pulmonary fibrosis