PXD060579

PXD060579 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Characterization of human CD34+ HSC-derived neutrophils without myeloid-derived immunosuppressive cell activity
Description	Neutrophils have limited utility as transfusion product due to their short lifespan. Culturing neutrophils from stem cell sources holds potential for fundamental and translational purposes. Here, we cultured CD34+ hematopoietic stem cell-derived neutrophils and compared them with peripheral blood neutrophils in terms of morphology, phenotype, and function. Our culture system resulted in 60% morphologically mature CD15+CD11b+CD16high neutrophils with most effector functions almost indistinguishable from blood neutrophils, confirmed by a high similarity in transcription and protein abundance patterns. While exhibiting strong microbial killing capacity and antibody-dependent cellular cytotoxicity against tumor cells, these cells were deficient in myeloid-derived suppressor cell activity. Upon dissecting the underlying mechanism, this deficiency in immunosuppressive activity correlated with their distinct granular composition in comparison to blood neutrophils. Taken together, our cultured neutrophils closely resemble blood neutrophils, offering a repository for fundamental research and a step towards an effective transfusion product without immunosuppressive activity.
HostingRepository	PRIDE
AnnounceDate	2025-08-13
AnnouncementXML	Submission_2025-08-13_05:17:37.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arie Hoogendijk
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-07 03:10:57	ID requested
⏵ 1	2025-08-13 05:17:38	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: MDSC, ADCC, proteomics, maturity, granules,Neutrophils, effector functions, transcriptomics, HSCs

submitter keyword: MDSC, ADCC, proteomics, maturity, granules,Neutrophils, effector functions, transcriptomics, HSCs

Contact List

Arie Johan Hoogendijk
contact affiliation	Sanquin Research, Amsterdam, NL
contact email	a.hoogendijk@sanquin.nl
lab head
Arie Hoogendijk
contact affiliation	Sanquin Research, Amsterdam
contact email	a.hoogendijk@sanquin.nl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD060579
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD060579

PRIDE project URI

Repository Record List

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